Pseudomonas Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2014.
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| Edición: | 1st ed. 2014. |
| Colección: | Methods in Molecular Biology,
1149 |
| Materias: |
Tabla de Contenidos:
- Gene Transfer: Transduction
- Gene Transfer: Transformation/Electroporation
- Gene transfer: Conjugation
- Pseudomonas Bacteriophage Isolation and Production
- Genotyping Methods
- Drug Susceptibility Testing by Dilution Methods
- Plate-based Assay for Swimming Motility in Pseudomonas aeruginosa
- Plate-based Assay for Swarming Motility in Pseudomonas aeruginosa
- Motility Assay: Twitching Motility
- Qualitative and Quantitative Assays for Flagellum-mediated Chemotaxis
- Microscopic Analysis: Morphotypes and Cellular Appendages
- Determination of Lipolytic Activities
- Elastinolytic and proteolytic enzymes
- In vitro Assays to Monitor the Activity of Pseudomonas aeruginosa Type III Secreted Proteins
- Cell Fractionation
- Characterization of Molecular Interactions using Isothermal Titration Calorimetry
- Proteomic Analysis
- Membrane Proteomics of Pseudomonas aeruginosa
- Construction of Pseudomonas Two-hybrid Libraries for High-throughput Assays
- Biosensors for Qualitative and Semi-quantitative Analysis of Quorum Sensing Signal Molecules
- LC-MS/MS Quantitative Analysis of Quorum Sensing Signal Molecules
- LC/MS/MS-Based Quantitative Assay for the Secondary Messenger Molecule, c-di-GMP
- Metabolic Footprinting: Extracellular Metabolomic Analysis
- Pyoverdine and Pyochelin Measurements
- Measurement of Phenazines in Bacterial Cultures
- Extraction and Measurement of NAD(P)+ and NAD(P)H
- Cyanide Measurements in Bacterial Culture and Sputum
- Monitoring Iron Uptake by Siderophores
- Exopolysaccharide Quantification
- Liquid Chromatography/Mass Spectrometry for the Identification and Quantification of Rhamnolipids
- LPS quantitation Procedures
- Monitoring Lectin Interaction with Carbohydrates
- Mining the Pseudomonas Genome
- Identification of Bacterial Small RNAs by RNA Sequencing
- Gene Amplification and qRT-PCR
- The Standard European Vector architecture (SEVA) plasmid toolkit
- Chromosomal integration of transcriptional fusions
- A Method to Capture Large DNA Fragments from Genomic DNA
- Transposon Mutagenesis
- Site-directed Mutagenesis and Gene Deletion using Reverse Genetics
- Signature Tagged Mutagenesis
- Construction of a Pseudomonas aeruginosa Genomic Bank
- Strategy for Genome Sequencing Analysis and Assembly for Comparative Genomics of Pseudomonas Genomes
- Promoter Fusions with Optical Outputs in Individual Cells and in Populations
- Chromatin Immunoprecipitation for ChIP-chip and ChIP-Seq
- Transcriptional Analysis of Pseudomonas aeruginosa Infected Caenorhabditis elegans
- Methods for Studying Biofilm Formation: Flow Cells and Confocal Laser Scanning Microscop
- Biofilm Formation in the 96-well Microtiter Plate
- Methods for Studying Biofilm Dispersal in Pseudomonas aeruginosa
- Pseudomonas aeruginosa PA14 Pathogenesis in Caenorhabditis Elegans
- Assessing Pseudomonas Virulence using a Non-mammalian Host Model: Dictyostelium Discoideum
- Assessing Pseudomonas virulence with non-mammalian host: Galleria mellonella
- Assessing Pseudomonas Virulence with the Non-mammalian Host: Arabidopsis Thaliana
- Assessing Pseudomonas aeruginosa Persister/Antibiotic Tolerant Cells
- Assessing Pseudomonas Virulence with Non-mammalian Host: Zebrafish
- Assessing Pseudomonas Virulence with a Non-mammalian Host: Drosophila Melanogaster
- Assessing Pseudomonas Virulence using Host Cells
- Assessing Pseudomonas aeruginosa Virulence and the Host Response using Murine Models of Acute and Chronic Lung Infection
- Assessing Pseudomonas Virulence using Mammalian Models: Acute Infection Model
- Burn Mouse Models.