|
|
|
|
| LEADER |
04139nam a22002775i 4500 |
| 001 |
000289466 |
| 005 |
20210901084236.0 |
| 007 |
cr nn 008mamaa |
| 008 |
151222s2016 xxu| s |||| 0|eng d |
| 020 |
|
|
|a 9781493931798
|
| 024 |
7 |
|
|a 10.1007/978-1-4939-3179-8
|2 doi
|
| 040 |
|
|
|a Sistema de Bibliotecas del Tecnológico de Costa Rica
|
| 245 |
1 |
0 |
|a P-Type ATPases :
|b Methods and Protocols /
|c edited by Maike Bublitz.
|
| 250 |
|
|
|a 1st ed. 2016.
|
| 260 |
# |
# |
|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2016.
|
| 300 |
|
|
|a XIV, 544 p. 107 illus., 51 illus. in color. :
|b online resource.
|
| 336 |
|
|
|a text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a computer
|b c
|2 rdamedia
|
| 338 |
|
|
|a online resource
|b cr
|2 rdacarrier
|
| 490 |
1 |
|
|a Methods in Molecular Biology,
|v 1377
|
| 505 |
0 |
|
|a An Introduction to P-type ATPase research -- Purification of Na,K-ATPase from Pig Kidney -- Preparation of Ca2+-ATPase1a Enzyme from Rabbit Sarcoplasmic Reticulum -- Isolation of H+/K+-ATPase-enriched Membrane Fraction from Pig Stomachs -- Overproduction of PiB-type ATPases -- Coordinated Overexpression in Yeast of a P4-ATPase and its Associated Cdc50 Subunit: The Case of the Drs2p/Cdc50p Lipid Flippase Complex -- The Plasma Membrane Ca2+-ATPase: Purification by Calmodulin Affinity Chromatography, and Reconstitution of the Purified Protein -- Expression of Na,K-ATPase and H,K-ATPase Isoforms With The Baculovirus Expression System -- Time-dependent Protein Thermostability Assay -- Colorimetric Assays of Na,K-ATPase -- ATPase Activity Measurements by an Enzyme-Coupled Spectrophotometric Assay -- Antimony-Phosphomolybdate ATPase Assay -- ATPase Activity Measurements using radiolabeled ATP -- Assaying P-type ATPases Reconstituted in Liposomes -- Coupling ratio for Ca2+ Transport by Calcium Oxalate Precipitation -- Calcium Uptake in Crude Tissue Preparation -- Measuring H+ Pumping and Membrane Potential Formation in Sealed Membrane Vesicle Systems -- Assay of Flippase Activity in Proteoliposomes using Fluorescent Lipid Derivatives -- The use of Metal Fluoride Compounds as Phosphate Analogs for Understanding the Structural Mechanism in P-type ATPases -- Phosphorylation/Dephosphorylation Assays -- Tryptophan Fluorescence Changes Related to Ca2+-ATPase Function -- Determination of the ATP Affinity of the Sarcoplasmic Reticulum Ca2+-ATPase by Competitive Inhibition of [g-32P]TNP-8N3-ATP Photolabeling. 23. Ca2+ Binding and Transport Studied with Ca2+/EGTA Buffers and 45Ca2+ 24. Assay of Copper Transfer and Binding to P1B-ATPases 25. Voltage Clamp Fluorometry of P-Type ATPases 26. Electrophysiological Measurements on Solid Supported Membranes 27. Electrophysiological Characterization of Na,K-ATPases Expressed in Xenopus laevis Oocytes Using Two-Electrode Voltage Clamping 28. Functional Studies of Na+,K+-ATPase using Transfected Cell Cultures 29. HPLC Neurotransmitter Analysis 30. Behavior Test Relevant to a2/a3Na+/K+-ATPase Gene Modified Mouse Models 31. Zebrafish Whole-mount in situ Hybridization Followed by Sectioning 32. Whole-mount Immunohistochemistry for Anti-F59 in Zebrafish Embryos (1-5 days Post Fertilization (dpf)) Canan Doganli 33. Cell-based Lipid Flippase Assay Employing Fluorescent Lipid Derivatives 34. Transient Expression of P-type ATPases in Tobacco Epidermal Cells 35. Lipid Exchange by Ultracentrifugation 36. Reconstitution of Na+, K+-ATPase in Nanodiscs 37. Crystallization of P-type ATPases by the High Lipid-detergent (HiLiDe) Method 38. Two-dimensional Crystallization of the Ca2+-ATPase for Electron Crystallography 39. Two-dimensional Crystallization of Gastric H+,K+-ATPase for Structural Analysis By Electron Crystallography 40. MD Simulations of P-type ATPases in a Lipid Bilayer System 41. Computational Classification of P-Type ATPases 42. Molecular Modeling of Fluorescent SERCA Biosensors 43. How to Compare, Analyze and Morph Between Crystal Structures of Different Conformations: The P-type ATPase Example Jesper L .
|
| 650 |
|
0 |
|a Cell membranes .
|
| 650 |
1 |
4 |
|a Membrane Biology.
|
| 700 |
1 |
|
|a Bublitz, Maike.
|e editor.
|
| 710 |
2 |
|
|a SpringerLink (Online service)
|
| 773 |
0 |
|
|t Springer eBooks
|