P-Type ATPases : Methods and Protocols /
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| Other Authors: | |
| Format: | eBook |
| Language: | English |
| Published: |
New York, NY :
Springer New York : Imprint: Humana,
2016.
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| Edition: | 1st ed. 2016. |
| Series: | Methods in Molecular Biology,
1377 |
| Subjects: |
Table of Contents:
- An Introduction to P-type ATPase research
- Purification of Na,K-ATPase from Pig Kidney
- Preparation of Ca2+-ATPase1a Enzyme from Rabbit Sarcoplasmic Reticulum
- Isolation of H+/K+-ATPase-enriched Membrane Fraction from Pig Stomachs
- Overproduction of PiB-type ATPases
- Coordinated Overexpression in Yeast of a P4-ATPase and its Associated Cdc50 Subunit: The Case of the Drs2p/Cdc50p Lipid Flippase Complex
- The Plasma Membrane Ca2+-ATPase: Purification by Calmodulin Affinity Chromatography, and Reconstitution of the Purified Protein
- Expression of Na,K-ATPase and H,K-ATPase Isoforms With The Baculovirus Expression System
- Time-dependent Protein Thermostability Assay
- Colorimetric Assays of Na,K-ATPase
- ATPase Activity Measurements by an Enzyme-Coupled Spectrophotometric Assay
- Antimony-Phosphomolybdate ATPase Assay
- ATPase Activity Measurements using radiolabeled ATP
- Assaying P-type ATPases Reconstituted in Liposomes
- Coupling ratio for Ca2+ Transport by Calcium Oxalate Precipitation
- Calcium Uptake in Crude Tissue Preparation
- Measuring H+ Pumping and Membrane Potential Formation in Sealed Membrane Vesicle Systems
- Assay of Flippase Activity in Proteoliposomes using Fluorescent Lipid Derivatives
- The use of Metal Fluoride Compounds as Phosphate Analogs for Understanding the Structural Mechanism in P-type ATPases
- Phosphorylation/Dephosphorylation Assays
- Tryptophan Fluorescence Changes Related to Ca2+-ATPase Function
- Determination of the ATP Affinity of the Sarcoplasmic Reticulum Ca2+-ATPase by Competitive Inhibition of [g-32P]TNP-8N3-ATP Photolabeling. 23. Ca2+ Binding and Transport Studied with Ca2+/EGTA Buffers and 45Ca2+ 24. Assay of Copper Transfer and Binding to P1B-ATPases 25. Voltage Clamp Fluorometry of P-Type ATPases 26. Electrophysiological Measurements on Solid Supported Membranes 27. Electrophysiological Characterization of Na,K-ATPases Expressed in Xenopus laevis Oocytes Using Two-Electrode Voltage Clamping 28. Functional Studies of Na+,K+-ATPase using Transfected Cell Cultures 29. HPLC Neurotransmitter Analysis 30. Behavior Test Relevant to a2/a3Na+/K+-ATPase Gene Modified Mouse Models 31. Zebrafish Whole-mount in situ Hybridization Followed by Sectioning 32. Whole-mount Immunohistochemistry for Anti-F59 in Zebrafish Embryos (1-5 days Post Fertilization (dpf)) Canan Doganli 33. Cell-based Lipid Flippase Assay Employing Fluorescent Lipid Derivatives 34. Transient Expression of P-type ATPases in Tobacco Epidermal Cells 35. Lipid Exchange by Ultracentrifugation 36. Reconstitution of Na+, K+-ATPase in Nanodiscs 37. Crystallization of P-type ATPases by the High Lipid-detergent (HiLiDe) Method 38. Two-dimensional Crystallization of the Ca2+-ATPase for Electron Crystallography 39. Two-dimensional Crystallization of Gastric H+,K+-ATPase for Structural Analysis By Electron Crystallography 40. MD Simulations of P-type ATPases in a Lipid Bilayer System 41. Computational Classification of P-Type ATPases 42. Molecular Modeling of Fluorescent SERCA Biosensors 43. How to Compare, Analyze and Morph Between Crystal Structures of Different Conformations: The P-type ATPase Example Jesper L .