Plant Transcription Factors : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Yuan, Ling. (Editor ), Perry, Sharyn E. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2011.
Edición:1st ed. 2011.
Colección:Methods in Molecular Biology, 754
Materias:
Tabla de Contenidos:
  • MADS & More: Transcription Factors that Shape the Plant
  • In Silico Mining and PCR Based Approaches to Transcription Factor Discovery in Non-Model Plants: Gene Discovery of the WRKY Transcription Factors in Conifers
  • Isolation of Plant Transcription Factors Using a Yeast One-Hybrid System
  • A Transposon Based Activation Tagging System for Gene Function Discovery in Arabidopsis
  • CRES-T, An Effective Gene Silencing System Utilizing Chimeric Repressors
  • Analysis of a Transcription Factor Using Transient Assay in Arabidopsis Protoplasts
  • Microarray-Based Identification of Transcription Factor Target Genes
  • Yeast Protein-Protein Interaction Assays and Screens
  • Mapping Functional Domains of transcription factors
  • Bimolecular fluorescence complementation as a tool to study interactions of regulatory proteins in plant protoplasts
  • Isolation of transcription factor complexes from Arabidopsis cell suspension cultures by tandem affinity purification
  • Assaying Transcription Factor Stability
  • How to assess the intercellular trafficking of transcription factors
  • SELEX (Systematic Evolution of Ligands by EXponential Enrichment), as a powerful tool for deciphering the protein-DNA interaction space
  • Footprinting and Missing Nucleoside Analysis of Transcription Factor-DNA Complexes
  • Chromatin immunoprecipitation to verify or to identify In Vivo protein-DNA interactions
  • Visualizing and characterizing In Vivo DNA-Binding Events and Direct Target Genes of Plant Transcription Factors
  • Mapping In Vivo Protein-DNA Interactions in Plants by DamID, a DNA Adenine Methylation-Based Method
  • Directed Evolution through DNA Shuffling for the Improvement and Understanding of Genes and Promoters.