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20201012091514.0 |
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cr nn 008mamaa |
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180618s2018 xxu| s |||| 0|eng d |
| 020 |
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|a 9781493978021
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| 024 |
7 |
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|a 10.1007/978-1-4939-7802-1
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Planarian Regeneration :
|b Methods and Protocols /
|c edited by Jochen C. Rink.
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| 250 |
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|a 1st ed. 2018.
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| 260 |
# |
# |
|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2018.
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| 300 |
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|a XI, 573 p. 92 illus., 56 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1774
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| 505 |
0 |
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|a Planarian Diversity and Phylogeny -- Stem Cells, Patterning, and Regeneration in Planarians-Self-Organization at the Organismal Scale -- The Ecology of Freshwater Planarians -- Planarians and the History of Animal Regeneration: Paradigm Shifts and Key Concepts in Biology -- Culturing Planarians in the Laboratory -- Total RNA Isolation from Planarian Tissues -- Small- and Large-Scale High Molecular Weight Genomic DNA Extraction from Planarians -- Planarian High Molecular Weight DNA Isolation by Spooling -- Basic Histological Techniques for Planarians -- Fixation, Processing, and Immunofluorescent Labeling of Whole Mount Planarians -- Immunohistochemistry on Paraffin-Embedded Planarian Tissue Sections -- Whole-Mount In Situ Hybridization of Planarians -- RNA In Situ Hybridization on Planarian Paraffin Sections -- General Principles of Planarian Embryogenesis and Its Analysis by In Situ Hybridization and Immunohistochemistry Methods -- Whole-Mount BrdU Staining with Fluorescence In Situ Hybridization in Planarians -- Detection of Apoptotic Cells in Planarians by Whole-Mount TUNEL -- Systemic RNA Interference in Planarians by Feeding of dsRNA Containing Bacteria -- RNA Interference in Planarians: Feeding and Injection of Synthetic dsRNA -- A Subtractive FACS Method for Isolation of Planarian Stem Cells and Neural Cells -- Clonal Analysis of Planarian Stem Cells by Subtotal Irradiation and Single-Cell Transplantation -- Tissue Transplantations in Planarians -- Live Imaging Planaria -- Processing Schmidtea mediterranea for Transmission Electron Microscopy: Classical and Microwave Techniques -- Tissue Extracts for Quantitative Mass Spectrometry of Planarian Proteins Using SILAC -- Quantification of Neurotransmitters from Intact and Regenerating Planarians Using UHPLC-MS/SRM Method.
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| 650 |
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0 |
|a Cell biology.
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| 650 |
1 |
4 |
|a Cell Biology.
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| 700 |
1 |
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|a Rink, Jochen C.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 900 |
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|a Libro descargado a ALEPH en bloque (proveniente de proveedor)
|