RNA Methylation : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Lusser, Alexandra. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2017.
Edición:1st ed. 2017.
Colección:Methods in Molecular Biology, 1562
Materias:
Tabla de Contenidos:
  • LC-MS Analysis of Methylated RNA
  • Comparative Analysis of Ribonucleic Acid Digests (CARD) by mass Spectrometry
  • Liquid Chromatography-Mass Spectrometry for Analysis of RNA Adenosine Methylation
  • Genome-Wide Location Analyses of N6-Methyladenosine Modifications (m6A-seq)
  • Mapping m6A at Individual-Nucleotide Resolution using Crosslinking and Immunoprecipitation (miCLIP)
  • Detection and Quantification of N6-Methyladenosine in Messenger RNA by TLC
  • Illustrating the Epitranscriptome at Nucleotide Resolution using Methylation-iCLIP (miCLIP)
  • Detection of 5-Methylcytosine in Specific Poly(A) RNAs by Bisulfite Sequencing
  • Transcriptome-Wide Detection of 5-Methylcytosine by Bisulfite Sequencing
  • Analysis of High-Throughput RNA Bisulfite Sequencing Data
  • Statistcal Methods for Transcriptome-Wide Analysis of RNA Methylation by Bisulfite
  • High-Throughput Mapping of 2’-O-Me Residues in RNA using Next-Generation Sequencing (Illumina RiboMethSeq Protocol)
  • RiboMeth-seq: Profiling of 2’-O-Me in RNA
  • In Silico Identification of RNA Modifications from High-Throughput Sequencing Data using HAMR
  • High-Throughput Small RNA Sequencing Enhanced by AlkB-Facilitated RNA de-Methylation (ARM-seq)
  • Transcriptome-Wide Mapping of N1-Methyladenosine Methylome
  • In Vitro Assays for RNA Methyltransferase Activity
  • Crosslinking Methods to Identify RNA Methyltransferase Targets In Vivo
  • Methylated mRNA Nucleotides as Regulators for Ribosomal Translation
  • Automated Chemical Solid-Phase Synthesis and Deprotection of 5-Hydroxymethylcytosine-Containing RNA.