RNAi and Plant Gene Function Analysis : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Kodama, Hiroaki. (Editor ), Komamine, Atsushi. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2011.
Edición:1st ed. 2011.
Colección:Methods in Molecular Biology, 744
Materias:
Tabla de Contenidos:
  • Overview of Plant RNAi
  • Caveat of RNAi in Plants: the Off-Target Effect
  • Plant Gateway Vectors for RNAi as a Tool for Functional Genomic Studies
  • Heat-Inducible RNAi for Gene Functional Analysis in Plants
  • Gene Function Analysis by Artificial MicroRNAs in Physcomitrella patens
  • Virus-Induced Gene Silencing in Ornamental Plants
  • Local RNA Silencing Mediated by Agroinfiltration
  • Direct Transfer of Synthetic Double-Stranded RNA into Protoplasts of Arabidopsis thaliana
  • Detection of Long and Short Double-Stranded RNAs
  • Quantitative Stem-Loop RT-PCR for Detection of MicroRNAs
  • Large Scale Sequencing of Plant Small RNAs
  • Computational Prediction of Plant miRNA Targets
  • Bisulfite Sequencing for Cytosine-Methylation Analysis in Plants
  • Using Nuclear Run-On Transcription Assays in RNAi Studies
  • Proteomic Analysis of RNA-Interference Induced Knockdown Plant
  • Comparative Analysis of Phosphoprotein Expression using 2D-DIGE.