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131031s2014 xxu| s |||| 0|eng d |
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|a 9781627036948
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024 |
7 |
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|a 10.1007/978-1-62703-694-8
|2 doi
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|a Sistema de Bibliotecas del TEC
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|a PIWI-Interacting RNAs :
|b Methods and Protocols /
|c edited by Mikiko C. Siomi.
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250 |
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|a 1st ed. 2014.
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2014.
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300 |
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|a XI, 249 p. 48 illus., 23 illus. in color. :
|b online resource.
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336 |
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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|a online resource
|b cr
|2 rdacarrier
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|a Methods in Molecular Biology,
|v 1093
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|a Chromatin Immunoprecipitation Assay of Piwi in Drosophila -- Drosophila Germline Stem Cell for in vitro Analyses of PIWI-Mediated RNAi -- RNAi and Overexpression of Genes in Ovarian Somatic Cells -- Making piRNAs in vitro -- A Framework for piRNA Cluster Manipulation -- Mass Spectrometric Analysis of 3’-End Methylation of piRNAs -- HITS-CLIP (CLIP-Seq) for Mouse Piwi Proteins -- Analysis of DNA Methylation in Mouse Testes -- Analysis of Small RNA-Guided Endonuclease Activity in Endogenous Piwi Protein Complexes from Mouse Testes -- Small RNA Library Construction from Minute Biological Samples -- Analysis of sDMA Modifications of PIWI Proteins -- Analyses of piRNA-Mediated Transcriptional Transposon Silencing in Drosophila: Nuclear Run-On Assay on Ovaries -- Combined RNA/DNA Fluorescence in situ Hybridization on Whole-Mount Drosophila Ovaries -- Fast and Accurate Method to Purify Small Non-Coding RNAs from Drosophila Ovaries -- Isolation of Zebrafish Gonads for RNA Isolation -- Small RNA Library Construction for High-Throughput Sequencing -- Analysis of Piwi-Loaded Small RNAs in Tetrahymena -- Effective Gene Knockdown in the Drosophila Germline by Artificial miRNA-Mimicking siRNAs -- Isolation and Bioinformatics Analyses of Small RNAs Interacting with Germ-Cell Specific Argonaute in Rice.
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|a Human genetics.
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|a Human Genetics.
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|a Siomi, Mikiko C.
|e editor.
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|a SpringerLink (Online service)
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|t Springer eBooks
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