PIWI-Interacting RNAs : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Siomi, Mikiko C. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2014.
Edición:1st ed. 2014.
Colección:Methods in Molecular Biology, 1093
Materias:
LEADER 02240nam a22002775i 4500
001 000290084
005 20210602105306.0
007 cr nn 008mamaa
008 131031s2014 xxu| s |||| 0|eng d
020 |a 9781627036948 
024 7 |a 10.1007/978-1-62703-694-8  |2 doi 
040 |a Sistema de Bibliotecas del TEC 
245 1 0 |a PIWI-Interacting RNAs :  |b Methods and Protocols /  |c edited by Mikiko C. Siomi. 
250 |a 1st ed. 2014. 
260 # # |a Totowa, NJ :  |b Humana Press :  |b Imprint: Humana,  |c 2014. 
300 |a XI, 249 p. 48 illus., 23 illus. in color. :  |b online resource. 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
490 1 |a Methods in Molecular Biology,  |v 1093 
505 0 |a Chromatin Immunoprecipitation Assay of Piwi in Drosophila -- Drosophila Germline Stem Cell for in vitro Analyses of PIWI-Mediated RNAi -- RNAi and Overexpression of Genes in Ovarian Somatic Cells -- Making piRNAs in vitro -- A Framework for piRNA Cluster Manipulation -- Mass Spectrometric Analysis of 3’-End Methylation of piRNAs -- HITS-CLIP (CLIP-Seq) for Mouse Piwi Proteins -- Analysis of DNA Methylation in Mouse Testes -- Analysis of Small RNA-Guided Endonuclease Activity in Endogenous Piwi Protein Complexes from Mouse Testes -- Small RNA Library Construction from Minute Biological Samples -- Analysis of sDMA Modifications of PIWI Proteins -- Analyses of piRNA-Mediated Transcriptional Transposon Silencing in Drosophila: Nuclear Run-On Assay on Ovaries -- Combined RNA/DNA Fluorescence in situ Hybridization on Whole-Mount Drosophila Ovaries -- Fast and Accurate Method to Purify Small Non-Coding RNAs from Drosophila Ovaries -- Isolation of Zebrafish Gonads for RNA Isolation -- Small RNA Library Construction for High-Throughput Sequencing -- Analysis of Piwi-Loaded Small RNAs in Tetrahymena -- Effective Gene Knockdown in the Drosophila Germline by Artificial miRNA-Mimicking siRNAs -- Isolation and Bioinformatics Analyses of Small RNAs Interacting with Germ-Cell Specific Argonaute in Rice. 
650 0 |a Human genetics. 
650 1 4 |a Human Genetics. 
700 1 |a Siomi, Mikiko C.  |e editor. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks