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02884nam a22003015i 4500 |
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151117s2016 xxu| s |||| 0|eng d |
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|a 9781493931156
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| 024 |
7 |
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|a 10.1007/978-1-4939-3115-6
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Plant Signal Transduction :
|b Methods and Protocols /
|c edited by Jose R. Botella, Miguel A. Botella.
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| 250 |
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|a 2nd ed. 2016.
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| 260 |
# |
# |
|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2016.
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| 300 |
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|a XV, 241 p. 41 illus., 25 illus. in color. :
|b online resource.
|
| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1363
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| 505 |
0 |
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|a Suppressor Screens in Arabidopsis -- Quantitative Reverse Transcription-qPCR-Based Gene Expression Analysis in Plants -- DNA-Binding Factor Target Identification by Chromatin Immunoprecipitation (ChIP) in Plants -- Identification of Genes Responsible for Natural Variation in Volatile Content Using Next Generation Sequencing Technology -- Micro-Tom Tomato as an Alternative Plant Model System: Mutant Collection and Efficient Transformation -- Culture of the Tomato Micro-Tom Cultivar in Greenhouse -- Virus-Induced Gene Silencing as a Tool to Study Tomato Fruit Biochemistry -- A Simplified and Rapid Method for the Isolation and Transfection of Arabidopsis Leaf Mesophyll Protoplasts for Large-Scale Applications -- Preparation of Epidermal Peels and Guard Cell Protoplasts for Cellular, Electrophysiological, and -Omics Assays of Guard Cell Function -- Application of Variable Angle Total Internal Reflection Fluorescence Microscopy to Investigate Protein Dynamics in Intact Plant Cells -- Immunoprecipitation of Plasma Membrane Receptor-Like Kinases for Identification of Phosphorylation Sites and Associated Proteins -- Yeast Three-Hybrid System for the Detection of Protein-Protein Interactions -- Cautions in Measuring In Vivo Interactions Using FRET and BiFC in Nicotiana benthamiana -- Analysis of Protein-Lipid Interactions Using Purified C2 Domains -- Assessing Kinase Activity in Plants with In-Gel Kinase Assays -- Analyses of Plant UDP-Dependent Glycosyltransferases to Identify Their Volatile Substrates Using Recombinant Proteins -- Competitive Index: Mixed Infection-Based Virulence Assays for Genetic Analysis in Pseudomonas syringae-Plant Interactions -- Simplified Assays for Evaluation of Resistance to Alternaria brassicicola and Turnip Mosaic Virus -- Simultaneous Determination of Plant Hormones by GC-TOF-MS.
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| 650 |
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0 |
|a Plant science.
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| 650 |
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0 |
|a Botany.
|
| 650 |
1 |
4 |
|a Plant Sciences.
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| 700 |
1 |
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|a Botella, Jose R.
|e editor.
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| 700 |
1 |
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|a Botella, Miguel A.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
|