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RNAi and Small Regulatory RNAs in Stem Cells : Methods and Protocols /

RNAi and Small Regulatory RNAs in Stem Cells : Methods and Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Zhang, Baohong. (Editor)
Format: eBook
Language:English
Published: New York, NY : Springer New York : Imprint: Humana, 2017.
Edition:1st ed. 2017.
Series:Methods in Molecular Biology, 1622
Subjects:
Cell biology.
Cell Biology.
Table of Contents:
  • RNAi Technique in Stem Cell Research: Current Status and Future Perspectives
  • RNAi and MicroRNA-Mediated Gene Regulation in Stem Cells
  • Forward RNAi Screens in Human Hematopoietic Stem Cells
  • Silencing of ATP11B by RNAi-Induced Changes in Neural Stem Cell Morphology
  • High-Efficiency Transfection of Glioblastoma Cells and a Simple Spheroid Migration Assay
  • Construction and Application of Random dsRNA Interference Library for Functional Genetic Screens in Embryonic Stem Cells
  • Efficient Depletion of Essential Gene Products for Loss-of-Function Studies in Embryonic Stem Cells
  • Regulation and/or Repression of Cholinergic Differentiation of Murine Embryonic Stem Cells Using RNAi Directed Against Transcription Factor L3/Lhx8
  • A Recessive Genetic Screen for Components of the RNA Interference Pathway Performed in Mouse Embryonic Stem Cells
  • RNAi Knockdown of Ape1 Gene in the Differentiation of Mouse Embryonic Stem Cells
  • An Efficient Transfection Method for Differentiation and Cell Proliferation of Mouse Embryonic Stem Cells
  • Implanting Glioblastoma Spheroids into Rat Brains and Monitoring Tumor Growth by MRI Volumetry
  • RNAi-Based Techniques for the Analysis of Gene Function in Drosophila Germline Stem Cells
  • In Vivo RNAi in the Drosophila Follicular Epithelium: Analysis of Stem Cell Maintenance, Proliferation, and Differentiation
  • A Phenotype-Based RNAi Screening for Ras-ERK/MAPK Signaling-Associated Stem Cell Regulators in C. elegans
  • Engineering Human Mesenchymal Stem Cells to Release Adenosine Using miRNA Technology
  • Efficient Gene Knockdowns in Mouse Embryonic Stem Cells Using MicroRNA-Based shRNAs
  • Using Quantitative Real-Time PCR to Detect MicroRNA Expression Profile During Embryonic Stem Cell Differentiation
  • Genetic Tools for Self-Organizing Culture of Mouse Embryonic Stem Cells Via Small Regulatory RNA-Mediated Technologies, CRISPR/Cas9 and Inducible RNAi
  • CRISPR-Cas9-Mediated Gene Editing in Mouse Spermatogonial Stem Cells.

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