Plant Chromatin Dynamics : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Bemer, Marian. (Editor ), Baroux, Célia. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2018.
Edición:1st ed. 2018.
Colección:Methods in Molecular Biology, 1675
Materias:
Tabla de Contenidos:
  • Section Overview: Profiling Developmentally and Environmentally Controlled Chromatin Reprogramming
  • Profiling DNA Methylation using Bisulfite Sequencing (BS-Seq)
  • Bisulfite Sequencing using Small DNA Amounts
  • Identification of Differentially Methylated Regions in the Genome of Arabidopsis thaliana
  • A Rapid and Efficient ChIP Protocol to Profile Chromatin Binding Proteins and Epigenetic Modifications in Arabidopsis
  • Sequential ChIP Protocol for Profiling Bivalent Epigenetic Modifications (ReChIP)
  • A Method to Identify Nucleolus-Associated Chromatin Domains (NADs)
  • Cell Type-Specific Profiling of Chromatin Modifications and Associated Proteins
  • Mapping of Histone Modifications in Plants by Tandem Mass Spectrometry
  • Histone H1 Purification and Post-Translational Modification Profiling by High–Resolution Mass Spectrometry
  • Profiling Nucleosome Occupancy by MNase-seq: Experimental Protocol and Computational Analysis
  • Identification of Open Chromatin Regions in Plant Genomes using ATAC-Seq
  • Section Overview: Unravelling the Complex Epigenetic Mechanisms that Regulate Gene Activity
  • Technical Review: A Hitchhiker’s Guide to Chromosome Conformation Capture
  • 3C in Maize and Arabidopsis
  • Profiling Histone Modifications in Synchronised Floral Tissues for Quantitative Resolution of Chromatin and Transcriptome Dynamics
  • De Novo Identification of sRNA Loci and Non-Coding RNAs by High-Throughput Sequencing
  • Identification of in planta Protein-Protein Interactions using IP-MS
  • RNA Immunoprecipitation Protocol to Identify Protein-RNA Interactions in Arabidopsis thaliana
  • In vitro Assays to Measure Histone Methyltransferase Activity using Different Chromatin Substrates
  • Identification of Parent-of-Origin-Dependent QTLs using Bulk-Segregant Sequencing (Bulk-Seq)
  • QTLepi mapping in Arabidopsis thaliana
  • Section Overview: a Compendium of Methods to Analyze the Spatial Organization of Plant Chromatin
  • Localisation of Chromatin Marks in Arabidopsis Early Embryos
  • Cell-type Specific Chromatin Analysis in Whole-mount Plant Tissues by Immunostaining
  • Measuring Dynamics of Histone Proteins by Photobleaching in Arabidopsis Roots
  • Fluorescence In Situ Hybridization (FISH) and Immunolabeling on 3D Preserved Nuclei
  • High-Affinity LNA/DNA Mixmer Probes for Detection of Chromosome-Specific Polymorphisms of 5S rDNA Repeats in Arabidopsis thaliana
  • A Method for Testing Random Spatial Model on Nuclear Object Distributions
  • Technical Review: Cytogenetic Tools for Studying Mitotic Chromosomes
  • Technical Review: Microscopy and Image Processing Tools to Analyse Plant Chromatin – practical Considerations
  • Automated 3D Gene Position Analysis using a Customized Imaris Plugin: XTFISHInsideNucleus
  • Quantitative 3D Analysis of Nuclear Morphology and Heterochromatin Organization from Whole Mount Plant Tissue using NucleusJ
  • Transmission Electron Microscopy Imaging to Analyse Chromatin Density Distribution at the Nanoscale Level. .