RNA Detection and Visualization : Methods and Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Gerst, Jeffrey E. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 2011.
Edition:1st ed. 2011.
Series:Methods in Molecular Biology, 714
Subjects:
Table of Contents:
  • Single Molecule Imaging of RNA In situ
  • FISH and Immunofluorescence Staining in Chlamydomonas
  • High Resolution Fluorescent In situ Hybridization in Drosophila
  • Localization and Anchorage of Maternal mRNAs to Cortical Structures of Ascidian Eggs and Embryos Using High Resolution In situ Hybridization
  • Visualization of mRNA Localization in Xenopus Oocytes
  • Visualization of mRNA Expression in the Zebrafish Embryo
  • High-Resolution Fluorescence In situ Hybridization to Detect mRNAs in Neuronal Compartments In vitro and In vivo
  • Localization of mRNA in Vertebrate Axons Using In situ Hybridization
  • Tiny Molecular Beacons for In vivo mRNA Detection
  • Delivery of Molecular Beacons for Live-Cell Imaging and Analysis of RNA
  • Genetically-Encoded Fluorescent Probes for Imaging Endogenous mRNA in Living Cells
  • Visualization of Induced RNA in Single Bacterial Cells
  • Visualizing mRNAs in Fixed and Living Yeast Cells
  • In vivo Visualization of RNA Using the U1A-Based Tagged RNA System
  • Visualizing Endogenous mRNAs in Living Yeast Using m-TAG, a PCR-Based RNA Aptamer Integration Method, and Fluorescence Microscopy
  • Imaging mRNAs in Living Mammalian Cells
  • Using the mRNA-MS2/MS2CP-FP System to Study mRNA Transport during Drosophila Oogenesis
  • Genome-Wide Analysis of RNA Extracted from Isolated Mitochondria
  • Analyzing mRNA Localization to the Endoplasmic Reticulum via Cell Fractionation
  • Isolation of mRNAs Encoding Peroxisomal Proteins from Yeast Using a Combined Cell Fractionation and Affinity Purification Procedure
  • Profiling Axonal mRNA Transport
  • RNA Purification from Tumor Cell Protrusions Using Porous Polycarbonate Filters
  • RNA-Binding Protein Immunopurification-Microarray (RIP-Chip) Analysis to Profile Localized RNAs
  • RaPID: An Aptamer-Based mRNA Affinity Purification Technique for the Identification of RNA and Protein Factors Present in Ribonucleoprotein Complexes
  • RIP: An mRNA Localization Technique
  • The Dual Use of RNA Aptamer Sequences for Affinity Purification and Localization Studies of RNAs and RNA-Protein Complexes
  • Identifying and Searching for Conserved RNA Localization Signals
  • Computational Prediction of RNA Structural Motifs Involved in Post Transcriptional Regulatory Processes.