Pseudogenes : Functions and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Poliseno, Laura. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2014.
Edición:1st ed. 2014.
Colección:Methods in Molecular Biology, 1167
Materias:
Tabla de Contenidos:
  • Computational Methods for Pseudogene Annotation Based on Sequence Homology.-  Computational Methods of Identification of Pseudogenes Based on Functionality: Entropy and GC Content
  • Computational Methods of Identification of Pseudogenes Based on Functionality: Hybridization of 18S rRNA and mRNA During Translation
  • Whole Genome Identification of Neutrally Evolving Pseudogenes Using the Evolutionary Measure dN/dS
  • Methods to Study the Occurrence and the Evolution of Pseudogenes Through a Phylogenetic Approach
  • Methods for Detecting Transcribed Pseudogenes: PCR on Regions of High Sequence Similarity Followed by Cloning and Sequencing,0 RNA Amplification for Pseudogene Detection Using RNA-Seq.- GENCODE Pseudogenes
  • Methods to Detect Transcribed Pseudogenes: RNA-Seq Discovery Allows Learning Through Features
  • Proteomics Techniques for the Detection of Translated Pseudogenes
  • Pseudogenes as Competitive Endogenous RNAs: Target Prediction and Validation
  • Pseudogenes: A Novel Source of Trans Acting Antisense RNAs
  • Pseudogene-Derived Endogenous siRNAs and Their Function
  • Methods to Study Translated Pseudogenes: In Vitro Translation, Fusion with a Tag/Reporter Gene, and Complementation Assay
  • Targeted and Robust Amplification of Mitochondrial DNA in the Presence of Nuclear Encoded Mitochondrial Pseudogenes Using Φ29 DNA Polymerases
  • Discrimination of Pseudogene and Parental Gene DNA Methylation Using Allelic Bisulphite Sequencing Mutational Analysis of CYP21A2 Gene and CYP21A1P Pseudogene: Long Range PCR on Genomic DNA
  • PMS2 Gene Mutational Analysis: RNA-Based Direct cDNA Sequencing to Circumvent Pseudogene Interference
  • Dealing with Pseudogenes in Molecular Diagnostics in the Next Generation Sequencing Era.