Opioid Receptors : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Spampinato, Santi M. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2015.
Edición:1st ed. 2015.
Colección:Methods in Molecular Biology, 1230
Materias:
Tabla de Contenidos:
  • Overview of Genetic Analysis of Human Opioid Receptors
  • Computational Structural Biology of Opioid Receptors
  • Analysis of Epigenetic Mechanisms Regulating Opioid Receptor Gene Transcription
  • Renilla Luciferase Reporter Assay to Study 3’UTR-Driven Post-Transcriptional Regulations of OPRM1
  • High-Throughput Gene Expression Profiling of Opioid-Induced Alterations in Discrete Brain Areas
  • Real-Time Imaging of Mu Opioid Receptors by Total Internal Reflection Fluorescence Microscopy
  • In Vivo Techniques to Investigate the Internalization Profile of Opioid Receptors
  • Monitoring Opioid Receptor Interaction in Living Cells by Bioluminescence Resonance Energy Transfer (BRET)
  • Bioluminescence Resonance Energy Transfer (BRET) to Detect the Interactions Between Kappa Opioid Receptor and Non-Visual Arrestins
  • Identification and Verification of Proteins Interacting with the Kappa Opioid Receptor (KOPR)
  • Detection of Mu Opioid Receptor (MOPR) and Its Glycosylation in Rat and Mouse Brains by Western Blot with Anti-µC, an Affinity-Purified Polyclonal Anti-MOPR Antibody
  • Immunohistochemical Analysis of Opioid Receptors in Peripheral Tissues
  • [35S]GTPgS-Autoradiography for Studies of Opioid Receptor Functionality
  • Fluorescence-Based, High-Throughput Assays for µ-Opioid Receptor Activation Using Membrane Potential-Sensitive Dye
  • Analysis of Potassium and Calcium Imaging to Assay the Function of Opioid Receptors
  • Electrophysiological Patch Clamp Assay to Monitor t he Action of Opioid Receptors
  • Skin-Nerve Preparation to Assay the Function of Opioid Receptors in Peripheral Endings of Sensory Neurons
  • Mechanical Nociception Measurement in Mice and Rats with Automated Von Frey Equipment
  • Detecting Zinc Release Induced by Mu-Opioid Receptor Agonists in Brain Slices
  • Opioid Receptors: Methods for Detection and Their Modes of Actions in the Eye
  • Evaluation of Murine Macrophage Cytokine Production After In Vivo Morphine Treatment
  • Measurement of Macrophage Toll Like Receptor 4 Expression After Morphine Treatment
  • The Role of Opioid Receptors in Migration and Wound Recovery In Vitro in Cultured Human Keratinocytes and Fibroblasts
  • Role of Opioid Receptors in the Reinstatement of Opioid-Seeking Behavior: An Overview
  • Analysis of Opioid-Seeking Reinstatement in the Rat
  • Induction of a High Alcohol Consumption in Rats and Mice: Role of Opioid Receptors
  • Evaluation of Social and Non-Social Behaviors Mediated by Opioids in Mouse Pups.