Quantitative Real-Time PCR : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Biassoni, Roberto. (Editor ), Raso, Alessandro. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2014.
Edición:1st ed. 2014.
Colección:Methods in Molecular Biology, 1160
Materias:
Tabla de Contenidos:
  • Twenty Years of qPCR: A Mature Technology?
  • Minimum Information Necessary for Quantitative Real-Time PCR Experiments
  • Selection of Reliable Reference Genes for RT-qPCR Analysis
  • Introduction to Digital PCR
  • mRNA and micro RNA Purity and Integrity: The Key to Success in Expression Profiling
  • Mediator Probe PCR: Detection of Real-Time PCR by Label Free Probes and a Universal Fluorogenic Reporter
  • Absolute Quantification of Viral DNA: The Quest for Perfection
  • A Multiplex Real-Time PCR-Platform Integrated into Automated Extraction Method for the Rapid Detection and Measurement of Oncogenic HPV Type-Specific Viral DNA Load from Cervical Samples
  • Real-Time PCR Detection of Mycoplasma pneumoniae in the Diagnosis of  Community-Acquired Pneumonia
  • A Sensible Technique to Detect Mollicutes Impurities in Human Cells Cultured in GMP Condition
  • Real-Time Quantification Assay to Monitor BCR-ABL1 Transcripts in Chronic Myeloid Leukemia
  • A Reliable Assay for Rapidly Defining Transplacental Metastasis Using Quantitative PCR
  • Circulating Cell-Free DNA in Cancer
  • Gene Expression Analysis by qPCR in Clinical Kidney Transplantation
  • Post-Transcriptional Regulatory Networks: From Expression Profiling to Integrative Analysis of mRNA and Micro RNA Data
  • Clinical Applications Using Digital PCR
  • Developing Non-Invasive Diagnosis for Single Gene Disorders: The Role of Digital PCR.