Single-Stranded DNA Binding Proteins : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Keck, James L. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2012.
Edición:1st ed. 2012.
Colección:Methods in Molecular Biology, 922
Materias:
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020 |a 9781627030328 
024 7 |a 10.1007/978-1-62703-032-8  |2 doi 
040 |a Sistema de Bibliotecas del Tecnológico de Costa Rica 
245 1 0 |a Single-Stranded DNA Binding Proteins :  |b Methods and Protocols /  |c edited by James L. Keck. 
250 |a 1st ed. 2012. 
260 # # |a Totowa, NJ :  |b Humana Press :  |b Imprint: Humana,  |c 2012. 
300 |a X, 259 p. 40 illus., 20 illus. in color. :  |b online resource. 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
490 1 |a Methods in Molecular Biology,  |v 922 
505 0 |a Functions of Single-Strand DNA-Binding Proteins in DNA Replication, Recombination and Repair.-Structural Diversity Based on Variability in Quaternary Association. A Case Study Involving Eubacterial and Related SSBs -- SB Binding to ssDNA Using Isothermal Titration Calorimetry -- SSB-DNA Binding Monitored by Fluorescence Intensity and Anisotropy -- Single Molecule Analysis of SSB Dynamics on Single Stranded DNA -- Sample Preparation Methods to Analyze DNA-induced Structural Changes in Replication Protein A (RPA) -- Structural Studies of SSB Interaction with RecO -- Investigation of Protein-Protein Interactions of Single-Stranded DNA-Binding Proteins by Analytical Ultracentrifugation -- Ammonium Sulfate Co-Precipitation of SSB and Interacting Proteins -- Analyzing Interactions Between SSB and Proteins by the Use of Fluorescence Anisotropy -- Far Western Blotting as a Rapid and Efficient Method for Detecting Interactions Between DNA Replication and DNA Repair Proteins -- Methods for Analysis of SSB/Protein Interactions by SPR -- Use of Native Gels to Measure Protein Binding to SSB -- Identification of Small Molecules That Disrupt SSB/Protein Interactions Using a High-Throughput Screen -- Detection of Post-translational Modifications of Replication Protein A -- Detecting Posttranslational Modification of Bacterial SSB Proteins -- Fluorescent SSB as a Reagentless Biosensor for Single-Stranded DNA -- Fluorescent Single-Stranded DNA-Binding Proteins Enable in vitro and in vivo Studies< -- Use of Fluorescently Tagged SSB Proteins in in vivo Localization Experiments. 
650 0 |a Proteins . 
650 1 4 |a Protein Science. 
650 2 4 |a Protein Structure. 
700 1 |a Keck, James L.  |e editor. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks