T-Cell Motility : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Verma, Navin Kumar. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2019.
Edición:1st ed. 2019.
Colección:Methods in Molecular Biology, 1930
Materias:
Tabla de Contenidos:
  • An Introduction to LFA-1/ICAM-1 Interactions in T-Cell Motility
  • Isolation of Human Peripheral Blood T Lymphocytes
  • A Laboratory Model to Study T-Cell Motility
  • Quantification of T-Cell Migratory Phenotypes using High Content Analysis
  • Live Cell Imaging and Analysis to Capture T-Cell Motility in Real-Time
  • Three-Dimensional Structured Illumination Microscopy (3D-SIM) to Dissect Signalling Cross-Talks in Motile T-Cells
  • Real-Time Impedance-Based Detection of LFA-1-Stimulated T-Cell Transwell Chemotaxis
  • Quantitative Real-Time PCR for Evaluating Transcriptional Changes in T-Lymphocytes
  • GapmeR-Mediated Gene Silencing in Motile T-Cells
  • Live Imaging of Resident T-Cell Migration in Human Lymphoid Tissue Slices using Confocal Microscopy
  • Phosphoprotein Enrichment for Protein Analysis in Motile T-Lymphocytes
  • Immunometabolomic Phenotyping of Motile T-Cells
  • Profiling Activity of Cellular Kinases in Migrating T-Cells
  • Biotinylation Assay to Determine LFA-1 Recycling in Motile T-Lymphocytes
  • A Protocol to Study T-Cell Signalling in an Immune Synapse by Microscopy
  • Enzyme-Linked Immunosorbent Assay for T-Cell Dependent Immunogenicity Assessment of Therapeutic Peptides
  • Modified Intravital Microscopy to Assess Vascular Health and T-Cell Motility
  • Computational Analysis of Protein-Protein Interactions in Motile T-Cells.