Streptococcus pneumoniae : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Iovino, Federico. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2019.
Edición:1st ed. 2019.
Colección:Methods in Molecular Biology, 1968
Materias:
Tabla de Contenidos:
  • Optimal Conditions for Streptococcus pneumoniae Culture: In Solid and Liquid Media
  • Electron Microscopy to Study the Fine Structure of the Pneumococcal Cell
  • Immunofluorescent Staining and High-Resolution Microscopy to Study the Pneumococcal Cell
  • Construction of Fluorescent Pneumococci for In Vivo Imaging and Labeling of the Chromosome
  • High-Resolution and Super-Resolution Immunofluorescent Microscopy Ex Vivo to Study Pneumococcal Interactions with the Host
  • Natural Genetic Transformation: A Direct Route to Easy Insertion of Chimeric Genes into the Pneumococcal Chromosome
  • Gene Expression Analysis in the Pneumococcus
  • Transcriptional Knockdown in Pneumococci using CRISPR Interference
  • Protein Expression Analysis by Western Blot and Protein-Protein Interactions
  • Mass Spectrometry to Study the Bacterial Proteome from a Single Colony
  • Bead-Based Flow Cytometric Cell Counting of Live and Dead Bacteria
  • In Vitro Adhesion, Invasion, and Transcytosis of Streptococcus pneumonia with Host Cells
  • Growing and Characterizing Biofilms Formed by Streptococcus pneumoniae
  • In Vivo Mouse Models to Study How Pneumococci Interact with the Host and Cause Invasive Pneumococcal Disease
  • Two-Photon Intravital Imaging of Leukocytes in the Trachea during Pneumococcal Infection
  • IVIS Spectrum CT to Image the Progression of Pneumococcal Infections In Vivo
  • The Pneumococcus and Its Critical Role in Public Health
  • The Epidemiology and Biostatistics of Pneumococcus.