Target Identification and Validation in Drug Discovery : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2019.
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| Edición: | 2nd ed. 2019. |
| Colección: | Methods in Molecular Biology,
1953 |
| Materias: |
Tabla de Contenidos:
- Using Functional Genetics in Haploid Cells for Drug Target Identification
- Unbiased Forward Genetic Screening with Chemical Mutagenesis to Uncover Drug-Target Interactions
- Integration of RNAi and Small Molecule Screens to Identify Targets for Drug Development
- CellProfiler and KNIME: Open Source Tools for High Content Screening
- In Silico Target Druggability Assessment: From Structural to Systemic Approaches
- In Silico Drug-Target Profiling
- Locus-Specific Knock-In of a Degradable Tag for Target Validation Studies
- Expression of Human VH Single Domains as Fc-Fusions in Mammalian Cells
- The NeuroSphere Assay (NSA) Applied to Neural Stem Cells (NSCs) and Cancer Stem Cells (CSCs)
- 3D-3 Tumor Models in Drug Discovery for Analysis of Immune Cell Infiltration
- Establishment and Analysis of a 3D Co-Culture Spheroid Model of Pancreatic Adenocarcinoma for Application in Drug Discovery
- In Vivo Pharmacology Models for Cancer Target Research
- Use of CRISPR/Cas9 for the Modification of the Mouse Genome
- Assessment of Gene Function of Mouse Innate Lymphoid Cells for In Vivo Analysis Using Retroviral Transduction
- Creation of PDX-Bearing Humanized Mice to Study Immuno-Oncology
- Immunophenotyping of Tissue Samples Using Multicolour Flow Cytometry
- Combined MicroRNA In Situ Hybridization and Immunohistochemical Detection of Protein Markers
- Tangential Flow Filtration with or without Subsequent Bind Elute Size Exclusion Chromatography for Purification of Extracellular Vesicles
- Chromogenic Tissue-Based Methods for Detection of Gene Amplifications (or Rearrangements) Combined with Protein Overexpression in Clinical Samples.