|
|
|
|
| LEADER |
02773nam a22002775i 4500 |
| 001 |
000291575 |
| 005 |
20210917115916.0 |
| 007 |
cr nn 008mamaa |
| 008 |
170314s2017 xxu| s |||| 0|eng d |
| 020 |
|
|
|a 9781493969401
|
| 024 |
7 |
|
|a 10.1007/978-1-4939-6940-1
|2 doi
|
| 040 |
|
|
|a Sistema de Bibliotecas del Tecnológico de Costa Rica
|
| 245 |
1 |
0 |
|a Synthetic Protein Switches :
|b Methods and Protocols /
|c edited by Viktor Stein.
|
| 250 |
|
|
|a 1st ed. 2017.
|
| 260 |
# |
# |
|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2017.
|
| 300 |
|
|
|a XI, 341 p. 59 illus., 31 illus. in color. :
|b online resource.
|
| 336 |
|
|
|a text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a computer
|b c
|2 rdamedia
|
| 338 |
|
|
|a online resource
|b cr
|2 rdacarrier
|
| 490 |
1 |
|
|a Methods in Molecular Biology,
|v 1596
|
| 505 |
0 |
|
|a Synthetic Protein Switches: Theoretical and Experimental Considerations -- Construction of Allosteric Protein Switches by Alternate Frame Folding and Intermolecular Fragment Exchange -- Construction of Protein Switches by Domain Insertion and Directed Evolution -- Catalytic Amyloid Fibrils that Bind Copper to Activate Oxygen -- Ancestral Protein Reconstruction and Circular Permutation for Improving the Stability and Dynamic Range of FRET Sensors -- Method for Developing Optical Sensors Using a Synthetic Dye-Fluorescent Protein FRET Pair and Computational Modelling and Assessment -- Rational Design and Applications of Semi-Synthetic Modular Biosensors: SNIFITs and LUCIDs -- Ultrasensitive Firefly Luminescent Intermediate-Based Protein-Protein Interaction Assay (FlimPIA) Based on the Functional Complementation of Mutant Firefly Luciferases -- Quantitative and Dynamic Imaging of ATM Kinase Activity -- Creation of Antigen-Dependent β-Lactamase Fusion Protein Tethered by Circularly Permuted Antibody Variable Domains -- Protein and Protease Sensing by Allosteric Derepression -- DNA-Specific Biosensors Based on Intramolecular β-Lactamase-Inhibitor Complex Formation -- Engineering and Characterizing Synthetic Protease Sensors and Switches -- Characterizing Dynamic Protein-Protein Interactions Using the Genetically Encoded Split Biosensor Assay Technique Split TEV -- Development of a Synthetic Switch to Control Protein Stability in Eukaryotic Cells with Light -- Light-Regulated Protein Kinases Based on the CRY2-CIB1 System -- Yeast-Based Screening System for the Selection of Functional Light-Driven K+ Channels -- Primer-Aided Truncation for the Creation of Hybrid Proteins -- Engineering Small Molecule Responsive Split-Protein Kinases -- Directed Evolution Methods to Rewire Signaling Networks.
|
| 650 |
|
0 |
|a Biotechnology.
|
| 650 |
1 |
4 |
|a Biotechnology.
|
| 700 |
1 |
|
|a Stein, Viktor.
|e editor.
|
| 710 |
2 |
|
|a SpringerLink (Online service)
|
| 773 |
0 |
|
|t Springer eBooks
|