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170302s2017 xxu| s |||| 0|eng d |
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|a 9781493968572
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| 024 |
7 |
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|a 10.1007/978-1-4939-6857-2
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Synthetic Antibodies :
|b Methods and Protocols /
|c edited by Thomas Tiller.
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| 250 |
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|a 1st ed. 2017.
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| 260 |
# |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2017.
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| 300 |
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|a XIV, 420 p. 102 illus., 74 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1575
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| 505 |
0 |
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|a Antibody Mimetics, Peptides, and Peptidomimetics -- Construction of a scFv Library with Synthetic, Non-Combinatorial CDR Diversity -- Enzymatic Assembly for scFv Library Construction -- Directed Evolution of Protein Thermal Stability Using Yeast Surface Display -- Whole Cell Panning with Phage Display -- Generating Conformation and Complex-Specific Synthetic Antibodies -- High-Throughput IgG Conversion of Phage Displayed Fab Antibody Fragments by AmplYFast -- Utilization of Selenocysteine for Site-Specific Antibody Conjugation -- Solubility Characterization and Imaging of Intrabodies Using GFP-Fusions -- Antibody Validation by Immunoprecipitation Followed by Mass Spectrometry Analysis -- Novel HPLC-Based Screening Method to Assess Developability of Antibody-Like Molecules -- Glycosylation Profiling of α/β T Cell Receptor Constant Domains Expressed in Mammalian Cells -- A Proximity-Based Assay for Identification of Ligand and Membrane Protein Interaction in Living Cells -- A Biotin Ligase-Based Assay for the Quantification of the Cytosolic Delivery of Therapeutic Proteins -- Data-Driven Antibody Engineering Using Genedata Biologics™ -- Selection of Aptamers Against Whole Living Cells: From Cell-SELEX to Identification of Biomarkers -- Rapid Selection of RNA Aptamers that Activate Fluorescence of Small Molecules -- An Enzyme-Linked Aptamer Sorbent Assay to Evaluate Aptamer Binding -- Incorporating Aptamers in the Multiple Analyte Profiling Assays (xMAP): Detection of C-Reactive Protein -- Transferring the Selectivity of a Natural Antibody into a Molecularly Imprinted Polymer -- Preparation of Molecularly Imprinted Microspheres by Precipitation Polymerization -- Generation of Janus Molecularly Imprinted Polymer Particles -- Surface Engineering of Nanoparticles to Create Synthetic Antibodies -- H5N1 Virus Plastic Antibody Based on Molecularly Imprinted Polymers -- Replacement of Antibodies in Pseudo-ELISAs: Molecularly Imprinted Nanoparticles for Vancomycin Detection -- Cell and Tissue Imaging with Molecularly Imprinted Polymers.
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| 650 |
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|a Immunology.
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| 650 |
1 |
4 |
|a Immunology.
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| 700 |
1 |
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|a Tiller, Thomas.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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