Split Inteins : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Mootz, Henning D. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2017.
Edición:1st ed. 2017.
Colección:Methods in Molecular Biology, 1495
Materias:
Tabla de Contenidos:
  • Affinity Purification of Proteins in Tag-Free Form: Split-Intein-Mediated Ultra-Rapid Purification (SIRP)
  • Purification of Microbially Expressed Recombinant Proteins via a Dual ELP Split Intein System
  • Intracellular Production of Cyclic Libraries with SICLOPPS
  • Recombinant Expression of Cyclotides Using Split Inteins
  • Ribosomal Synthesis of Thioether-Bridges Bicyclic Peptides
  • Preparation of Semisynthetic Peptides Macrocycles Using Split Inteins
  • Semisynthesis of Membrane-Attached Proteins Using Split Inteins
  • Protein Chemical Modification Inside Living Cells Using Split Inteins
  • Segmental Isotopic Labeling of Proteins for NMR Study Using Intein Technology
  • Segmental Isotope Labeling of Insoluble Proteins for Solid-State NMR by Protein Trans-Splicing
  • Split-Intein Triggered Protein Hydrogels
  • A Recessive Pollination Control System for Wheat-Based on Intein-Mediated Protein Splicing
  • Conditional Toxin Splicing Using a Split Intein System
  • Photocontrol of the Src Kinase in mammalian Cells with a Photocaged Intein
  • LOV2-Controlled Photoactivation of Protein Trans-Splicing
  • A Cassette Approach for the Identification of Intein Insertion Sites
  • Computational Prediction of New Intein Split Sites.