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160926s2017 xxu| s |||| 0|eng d |
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|a 9781493963430
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| 024 |
7 |
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|a 10.1007/978-1-4939-6343-0
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Synthetic DNA :
|b Methods and Protocols /
|c edited by Randall A. Hughes.
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| 250 |
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|a 1st ed. 2017.
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| 260 |
# |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2017.
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| 300 |
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|a XIII, 248 p. 74 illus., 60 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1472
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| 505 |
0 |
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|a A Guide to Using STITCHER for Overlapping Assembly PCR Applications -- Synthetic Gene Design Using Codon Optimization On-Line (COOL) -- Shuffle Optimizer: A Program to Optimize DNA Shuffling for Protein Engineering -- Simple Cloning by Prolonged Overlap Extension PCR with Application to the Preparation of Large-Size Random Gene Mutagenesis Library in Escherichia coli -- SpeedyGenes: Exploiting an Improved Gene Synthesis Method for the Efficient Production of Error-corrected, Synthetic Protein Libraries for Directed Evolution -- BASIC – A Simple and Accurate Modular DNA Assembly Method -- Enzymatic Synthesis of Single Stranded Clonal Pure Oligonucleotides -- Rapid Assembly of DNA via Ligase Cycling Reaction (LCR) -- PaperClip: A Simple Method for Flexible Multi-part DNA Assembly -- The Polymerase Step Reaction (PSR) Method for Gene and Library Synthesis -- Clonetegration Using OSIP Plasmids – One Step DNA Assembly and Site Specific Genomic Integration in Bacteria -- Generation of DNA Constructs Using the Golden GATEway Cloning Method -- Gene Deletion by Synthesis in Yeast -- Efficient Assembly of DNA Using Yeast Homologous Recombination (YHR) -- Simultaneous Removal of Multiple DNA Segments by Polymerase Chain Reactions -- Rapid Construction of Recombinant Plasmids by QuickStep-Cloning -- Immobilized MutS-mediated Error Removal of Microchip-synthesized DNA -- Selection of Error-less Synthetic Genes in Yeast.
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| 650 |
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|a Human genetics.
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| 650 |
1 |
4 |
|a Human Genetics.
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| 700 |
1 |
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|a Hughes, Randall A.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 900 |
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|a Libro descargado a ALEPH en bloque (proveniente de proveedor)
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