Signal Transduction Immunohistochemistry : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Kalyuzhny, Alexander E. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2011.
Edición:1st ed. 2011.
Colección:Methods in Molecular Biology, 717
Materias:
Tabla de Contenidos:
  • Overview of the Generation, Validation, and Application of Phosphosite-Specific Antibodies
  • Selection and Validation of Antibodies for Signal Transduction Immunohistochemistry
  • An Overview of Western Blotting for Determining Antibody Specificities for Immunohistochemistry
  • Optimized Protocol to Make Phospho-Specific Antibodies that Work
  • Methodology and Technology for Stabilization of Specific States of Signal Transduction Proteins
  • An Enhanced Antigen Retrieval Protocol for Immunohistochemical Staining of Formalin-Fixed, Paraffin-Embedded Tissues
  • Imaging Techniques in Signal Transduction IHC
  • Practical Considerations of Image Analysis and Quantification of Signal Transduction IHC Staining
  • Flow Cytometric Analysis of Cell Signaling Proteins
  • CytoSys: A Tool for Extracting Cell Cycle-Related Expression Dynamics from Static Data
  • Signaling Events Initiated by Kappa Opioid Receptor Activation: Quantification and Immunolocalization Using Phospho-Selective KOR, p38-MAPK, and KIR 3.1 Antibodies
  • Immunohistochemical Assessment of Signal Transduction and Cell-Cycle Networks in Neural Tumors
  • Novel Multi-Color Immunofluorescence Technique Using Primary Antibodies Raised in the Same Host Species
  • Activation and Differentiation of Mesenchymal Stem Cells
  • Double In situ Detection of Sonic Hedgehog mRNA and pMAPK Protein in Examining the Cell Proliferation Signaling Pathway in Mouse Embryo
  • Identifying Intracellular Sites of Eicosanoid Lipid Mediator Synthesis with EicosaCell Assays
  • Absorption Control in Immunohistochemistry Using Phospho-Peptides Immobilized on Magnetic Beads.