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01774nam a22002895i 4500 |
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121227s1987 xxu| s |||| 0|eng d |
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|a 9781461597995
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| 024 |
7 |
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|a 10.1007/978-1-4615-9799-5
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 100 |
1 |
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|a Walker, John M.
|e author.
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| 245 |
1 |
0 |
|a Techniques in Molecular Biology :
|b Volume 2 /
|c by John M. Walker, Wim Gaastra.
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| 250 |
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|a 1st ed. 1987.
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| 260 |
# |
# |
|a New York, NY :
|b Springer US :
|b Imprint: Springer,
|c 1987.
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| 300 |
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|a IV, 332 p. 16 illus. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 505 |
0 |
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|a 1. Micromethods in Protein Chemistry -- 2. Peptide Synthesis -- 3. Affinity Chromatography -- 4. The Enzyme Linked Immunosorbent Assay (ELISA) -- 5. Monoclonal Antibodies -- 6. Plasmid Derived Cloning Vectors -- 7. The Ti-Plasmid of Agrobacterium tumefaciens as a Tool for Genetic Engineering in Plants -- 8. Cloning in Yeast -- 9. Cloning in Bacteriophage Lambda -- 10. The Dot-Blot Technique -- 11. DNA-mediated Gene Transfer into Mammalian Cells -- 12. In vitro Transcription -- 13. Chemical Synthesis of Oligodeoxyribonucleotides -- 14. Targeted Mutagenesis of Cloned DNA -- 15. DNA Footprinting and Related Techniques for Analysing Protein-DNA Interactions -- 16. Autoradiography and Fluorography -- 17. Computer Applications to Studying DNA -- List of Contributors.
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| 650 |
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0 |
|a Biochemistry.
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| 650 |
1 |
4 |
|a Biochemistry, general.
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| 650 |
2 |
4 |
|a Science, Humanities and Social Sciences, multidisciplinary.
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| 700 |
1 |
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|a Gaastra, Wim.
|e author.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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