The Inflammasome : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: De Nardo, Christine M. (Editor ), Latz, Eicke. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2013.
Edición:1st ed. 2013.
Colección:Methods in Molecular Biology, 1040
Materias:
Tabla de Contenidos:
  • Assessing b-amyloid-induced NLRP3 Inflammasome Activation in Primary Microglia
  • Activating the NLRP3 Inflammasome using the Amyloidogenic Peptide IAPP
  • Assessment and Quantification of Crystal-induced Lysosomal Damage
  • Assessment of Inflammasome Activation in Primary Human Immune Cells
  • NLRP3 Inflammasome Activation and Cytotoxicity Induced by particulate Adjuvants
  • Measuring Inflammasome Activation in Response to Bacterial Infection
  • Detection of Pyroptosis by Measuring Released Lactate Dehydrogenase Activity
  • ASC Speck Formation as a Read-out for Inflammasome Activation
  • Immunoblotting for Active Caspase-1
  •  Inflammasome Activation and Inhibition in Primary Murine Bone Marrow-Derived Cells, and Assays for IL-1α, IL-1β and Caspase-1
  • Reconstituting the NLRP1 Inflammasome in vitro
  • Assessing ATP Binding and Hydrolysis by NLR Proteins
  • Blue Native Polyacrylamide Gel Electrophoresis to Monitor Inflammasome Assembly and Composition
  • Analysis of Microbiota Alterations in Inflammasome-deficient Mice
  • Quantification of Adipose Tissue Leukocytosis in Obesity
  • In vivo Evaluation of Neutrophil Recruitment in Response to Sterile Particulates.