Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Warscheid, Bettina. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2014.
Edición:1st ed. 2014.
Colección:Methods in Molecular Biology, 1188
Materias:
Tabla de Contenidos:
  • Twelve Years of Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)
  • Stable Isotope Labeling by Amino Acids Applied to Bacterial Cell Culture
  • SILAC Labeling of Yeast for the Study of Membrane Protein Complexes
  • Whole Proteome Analysis of the Protozoan Parasite Trypanosoma brucei using Stable Isotope Labeling by Amino Acids in Cell Culture and Mass Spectrometry
  • Stable Isotope Labeling by Amino Acids in Cultured Primary Neurons
  • SILAC and Alternatives in Studying Cellular Proteomes of Plants
  • In Vivo Stable Isotope Labeling by Amino Acids in Drosophila melanogaster
  • Stable Isotope Labeling for Proteomic Analysis of Tissues in Mouse
  • Identification of Novel Protein Functions and Signaling Mechanisms by Genetics and Quantitative Phosphoproteomics in Caenorhabditis elegans
  • SILAC-based Temporal Phosphoproteomics
  • Global Ubiquitination Analysis by SILAC in Mammalian Cells
  • Quantifying In Vivo, Site-specific Changes in Protein Methylation with SILAC
  • Applying SILAC for the Differential Analysis of Protein Complexes
  • Defining Dynamic Protein Interactions Using SILAC-based Quantitative Mass Spectrometry
  • Identifying Nuclear Protein-Protein Interactions using GFP Affinity Purification and SILAC-based Quantitative Mass Spectrometry
  • Analyzing the Protein Assembly and Dynamics of the Human Spliceosome with SILAC
  • Identification and Validation of Protein-Protein Interactions by Combining Co-immunoprecipitation, Antigen Competition, and Stable Isotope Labeling
  • Protein Correlation Profiling-SILAC to Study Protein-Protein Interactions
  • Autophagosomal Proteome Analysis by Protein Correlation Profiling-SILAC
  • Design and Application of Super-SILAC for Proteome Quantification
  • Proteomics Meets Genetics: SILAC Labeling of Drosophila melanogaster Larvae and Cells for In Vivo Functional Studies
  • Analysis of Secreted Proteins using SILAC
  • Identification of MicroRNA Targets by Pulsed SILAC
  • MaxQuant for In-Depth Analysis of Large SILAC Datasets.