Synthetic Messenger RNA and Cell Metabolism Modulation : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Rabinovich, Peter M. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2013.
Edición:1st ed. 2013.
Colección:Methods in Molecular Biology, 969
Materias:
Tabla de Contenidos:
  • Cell Engineering with Synthetic Messenger RNA
  • In vitro Transcription of Long RNA Containing Modified Nucleosides
  • HPLC Purification of in vitro Transcribed Long RNA
  • Synthetic mRNAs with Superior Translation and Stability Properties
  • Non-Viral, Cationic Lipid-Mediated Delivery of mRNA
  • Light-Induced mRNA Transfection
  • Engineering B Cells with mRNA
  • Programming Human Dendritic Cells with mRNA
  • Large Volume Flow Electroporation of mRNA: Clinical Scale Process
  • Retrovirus-Based mRNA Transfer for Transient Cell Manipulation
  • mRNA Delivery to Human Dendritic Cells by Recombinant
  • Non-Viral RNA Transfection to Transiently Modify T Cells with Chimeric Antigen Receptors for Adoptive Therapy
  • Natural Killer Cell Reprogramming with Chimeric Immune Receptors
  • Reprogramming to Pluripotency and Differentiation of Cells with Synthetic mRNA
  • Anti-Tumor Vaccination with Synthetic mRNA: Strategies for in vitro and in vivo Preclinical Studies
  • Mannosylated and Histidylated LPR Technology for Vaccination with Tumor Antigen mRNA
  • Analysis of Surviving-Specific T Cells in Breast Cancer Patients Using Human DCs Engineered with Survivin mRNA
  • mRNA electroporation as a Tool for Immunomonitoring
  • mRNA PCR-Based Epitope Chase Method.