The Mitotic Spindle : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Chang, Paul. (Editor ), Ohi, Ryoma. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2016.
Edición:1st ed. 2016.
Colección:Methods in Molecular Biology, 1413
Materias:
Tabla de Contenidos:
  • Using Fluorescence Microscopy to Study Mitosis
  • Using Photoactivatable GFP to Study Microtubule Dynamics and Chromosome Segregation
  • Purification and Fluorescent Labeling of Tubulin from Xenopus Laevis Egg Extracts
  • Measuring the Effects of Microtubule-Associated Proteins on Microtubule Dynamics In Vitro
  • Imaging and Quantifying the Dynamics of γ-Tubulin at Microtubule Minus Ends in Mitotic Spindles
  • Visualizing and Analyzing Branching Microtubule Nucleation Using Meiotic Xenopus Egg Extracts and TIRF Microscopy
  • Encapsulation of Xenopus Egg and Embryo Extract Spindle Assembly Reactions in Synthetic Cell-Like Compartments with Tunable Size
  • In Vitro Kinetochore Assembly
  • Biochemical and Structural Analysis of Kinetochore Histone-Fold Complexes
  • Measuring Kinetochore-Microtubule Attachment Stability in Cultured Cells
  • Studying Kinetochores In Vivo Using FLIM-FRET
  • Purification of Fluorescently Labeled Saccharomyces Cerevisiae Spindle Pole Bodies
  • A Cell-Free System for Real-Time Analyses of Centriole Disengagement and Centriole to Centrosome Conversion
  • Assays to Study Mitotic Centrosome and Spindle Pole Assembly and Regulation
  • Analyzing Spindle Positioning Dynamics in Cultured Cells
  • Quantification of Mitotic Chromosome Alignment
  • Imaging Mitosis in the Moss Physcomitrella Patens
  • Small Molecule Approach to Study the Function of Mitotic Kinesins
  • Identification and Characterization of Mitotic Spindle-Localized Transcripts
  • Probing Mitosis by Manipulating the Interactions of Mitotic Regulator Proteins Using Rapamycin-Inducible Dimerization
  • Studying Kinetochore Kinases
  • Engineering and Functional Analysis of Mitotic Kinases through Chemical Genetics
  • Using Cell Culture Models of Centrosome Amplification to Study Centrosome Clustering in Cancer
  • Generation and Purification of Tetraploid Cells
  • Anti-Microtubule Drugs.