Single Molecule Analysis : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Peterman, Erwin J. G. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2018.
Edición:2nd ed. 2018.
Colección:Methods in Molecular Biology, 1665
Materias:
Tabla de Contenidos:
  • Introduction to Optical Tweezers: Background, System Designs, and Commercial Solutions
  • RNA Unzipping and Force Measurements with a Dual Optical Trap
  • Protein Tethering for Folding Studies
  • Combining Structure-Function and Single-Molecule Studies on Cytoplasmic Dynein
  • A Brief Introduction to Single-Molecule Fluorescence Methods
  • Fluorescent Labeling of Proteins
  • Single-Molecule Imaging of Escherichia coli Trans-Membrane Proteins
  • Single-Molecule Fluorescence Microscopy in Living Caenorhabditis elegans
  • Purification and Application of a Small Actin Probe for Single-Molecule Localization Microscopy
  • Fluorescence Microscopy of Nanochannel-Confined DNA
  • Use of Single Molecule Fluorescence Polarization Microscopy to Study Protein Conformation and Dynamics of Kinesin
  • Microtubule Complexes
  • Single Molecule FRET Analysis of DNA Binding Proteins
  • Atomic Force Microscopy: An Introduction
  • Imaging of DNA and Protein by SFM and Combined SFM-TIRF Microscopy
  • Atomic Force Microscopy of Protein Shells: Virus Capsids and Beyond
  • Combined Magnetic Tweezers and Micro-Mirror Total Internal Reflection Fluorescence Microscope for Single Molecule Manipulation and Visualization
  • Tethered Particle Motion: An Easy Technique for Probing DNA Topology and Transcription Factor Interactions
  • Single-Molecule Measurements Using Acoustic Force Spectroscopy (AFS)
  • Repurposing a Benchtop Centrifuge for High-Throughput Single-Molecule Force Spectroscopy. .