Shotgun Proteomics : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Martins-de-Souza, Daniel. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2014.
Edición:1st ed. 2014.
Colección:Methods in Molecular Biology, 1156
Materias:
Tabla de Contenidos:
  • Survey of Shotgun Proteomics
  • LC-MALDI-TOF/TOF for Shotgun Proteomics
  • Fully Automatable Multidimensional Reversed-Phase Liquid Chromatography with Online Tandem Mass Spectrometry
  • GeLC-MS/MS Analysis of Complex Protein Mixtures
  • IPG Strip-Based Peptide Fractionation for Shotgun Proteomics
  • SILAC Yeast: From Labeling to Comprehensive Proteome Quantification
  • Analysis of Proteome Dynamics in Mice by Isotopic Labeling
  • Stable Isotope Labeling in Mammals (SILAM)
  • Analysis of Individual Protein Turnover in Live Animals on a Proteome-Wide Scale
  • Determining Protein Subcellular Localization in Mammalian Cell Culture with Biochemical Fractionation and iTRAQ 8-Plex Quantification
  • Brain Quantitative Proteomics Combining GeLC-MS and Isotope-Coded Protein Labeling (ICPL)
  • Employing TMT Quantification in a Shotgun-MS Platform
  • Employing TMT Quantification in Shotgun-MS Proteomic Analysis: A Focus on Skeletal Muscle
  • Spectral Counting Label-Free Proteomics
  • Quantification of Proteins by Label Free LC-MSE
  • Bioinformatics for Proteomics: Opportunities at the Interface Between the Scientists, Their Experiments and the Community
  • Identification of DNA Damage Checkpoint-Dependent Protein Interactions in Saccharomyces cerevisiae Using Quantitative Mass Spectrometry
  • Application of Shotgun Proteomics for Discovery-Driven Protein-Protein Interaction
  • Mapping Protein Complexes Using Covalently Linked Antibodies and Isobaric Mass Tags
  • Biomarker Verification Using Selected Reaction Monitoring and Shotgun Proteomics
  • Use of Universal Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)-Based Selected Reaction Monitoring (SRM) Approach for Verification of Breast Cancer-Related Protein Markers
  • The Secretome Analysis by High Throughput Proteomics and Multiple Reaction Monitoring (MRM)
  • Preparation of Heteroelement-Incorporated and Stable Isotope-Labeled Protein Standards for Quantitative Proteomics
  • One-Source Peptide/Phosphopeptide Ratio Standards for Accurate and Site-Specific Determination of the Degree of Phosphorylation
  • Quantitative Glycoproteomics for N-Glycoproteome Profiling
  • A Practical Recipe to Survey Phosphoproteomes
  • Quantitation of the Phosphoproteome Using the Library Assisted eXtracted Ion Chromatogram (LAXIC) Strategy
  • Fast, Efficient and Quality-Controlled Phosphopeptide Enrichment from Minute Sample Amounts Using Titanium Dioxide
  • Quantifying Small Molecule-Induced Changes in Cellular Protein Expression and Post-Translational Modifications Using Isobaric Mass Tags
  • Analysis of Protein Structure by Crosslinking Combined with Mass Spectrometry
  • Top Down Proteomics by Means of Orbitrap Mass Spectrometry.