Single Molecule Analysis : Methods and Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Peterman, Erwin J. G. (Editor), Wuite, Gijs J. L. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 2011.
Edition:1st ed. 2011.
Series:Methods in Molecular Biology, 783
Subjects:
Table of Contents:
  • Introduction to Optical Tweezers: Background, System Designs, and Commercial Solutions
  • Optical Trapping and Unfolding of RNA
  • DNA Unzipping and Force Measurements with a Dual Optical Trap
  • Probing the Force Generation and Stepping Behavior of Cytoplasmic Dynein
  • A Brief Introduction to Single-molecule Fluorescence Methods
  • Fluorescent Labeling of Proteins
  • Fluorescence Imaging of Single Kinesin Motors on Immobilized Microtubules
  • Exploring Protein Superstructures and Dynamics in Live Bacterial Cells using Single-molecule and Superresolution Imaging
  • Fluorescence Microscopy of Nanochannel-confined DNA
  • Fluorescence Correlation Spectroscopy
  • Introduction to Atomic Force Microscopy
  • Sample Preparation for SFM Imaging of DNA, Proteins, and DNA-protein Complexes
  • Single Molecule Protein Unfolding and Refolding using Atomic Force Microscopy
  • How to Perform a Nanoindentation Experiment on a Virus
  • Magnetic Tweezers for Single-molecule Manipulation
  • Probing DNA topology using Tethered Particle Motion.