Stem Cell Transcriptional Networks : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Kidder, Benjamin L. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2014.
Edición:1st ed. 2014.
Colección:Methods in Molecular Biology, 1150
Materias:
Tabla de Contenidos:
  • Efficient Library Preparation for Next-Generation Sequencing Analysis of Genome-Wide Epigenetic and Transcriptional Landscapes in Embryonic Stem Cells
  • Analysis of Next-Generation Sequencing Data Using Galaxy
  • edgeR for Differential RNA-seq and ChIP-seq Analysis: An Application to Stem Cell Biology
  • Use Model-based Analysis of ChIP-Seq (MACS) to Analyze Short Reads Generated by Sequencing Protein-DNA Interactions in Embryonic Stem Cells
  • Spatial Clustering for Identification of ChIP-Enriched Regions (SICER) to Map Regions of Histone Methylation Patterns in Embryonic Stem Cells
  • Identifying Stem Cell Gene Expression Patterns and Phenotypic Networks with AutoSOME
  • Visualization and Clustering of High-Dimensional Transcriptome Data Using GATE
  • Interpreting and Visualizing ChIP-seq Data with the seqMINER Software
  • A Description of the Molecular Signatures Database (MSigDB) Website
  • Use of Genome-Wide RNAi Screens to Identify Regulators of Embryonic Stem Cell Self-Renewal and Pluripotency
  • Correlating Histone Modification Patterns with Gene Expression Data During Hematopoiesis
  • In Vitro Maturation and In Vitro Fertilization of Mouse Oocytes and Preimplantation Embryo Culture
  • Derivation and Manipulation of Trophoblast Stem Cells from Mouse Blastocysts
  • Conversion of Epiblast Stem Cells to Embryonic Stem Cells Using Growth Factors and Small Molecule Inhibitors
  • Generation of Induced Pluripotent Stem Cells Using Chemical Inhibition and Three Transcription Factors
  • Transdifferentiation of Mouse Fibroblasts and Hepatocytes to Functional Neurons
  • Direct Lineage Conversion of Pancreatic Exocrine to Endocrine Beta-Cells In Vivo with Defined Factors
  • Direct Reprogramming of Cardiac Fibroblasts to Cardiomyocytes Using MicroRNAs
  • Reprogramming Somatic Cells into Pluripotent Stem Cells Using miRNAs.