cDNA Libraries : Methods and Applications /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Lu, Chaofu. (Editor), Browse, John. (Editor), Wallis, James G. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 2011.
Edition:1st ed. 2011.
Series:Methods in Molecular Biology, 729
Subjects:
Table of Contents:
  • Isolation of Fungal Infection Structures from Plant Tissue by Flow Cytometry for Cell-Specific Transcriptome Analysis
  • Preparation of a High Quality cDNA Library from a Single-Cell Quantity of mRNA Using Chum-RNA
  • Construction of a Full-Length cDNA Library from Castor Endosperm for High-throughput Functional Screening
  • Full-Length Transcriptome Analysis Using a Bias-Free cDNA Library Prepared with the Vector-Capping Method
  • Construction of Improved Yeast Two-Hybrid Libraries
  • Normalization of Full-Length Enriched cDNA
  • Bioinformatic Methods for Finding Differentially Expressed Genes in cDNA Libraries, Applied to the Identification of Tumour Vascular Targets
  • Enzymatic Production of RNAi Libraries from cDNAs and High-Throughput Selection of Effective shRNA Expression Constructs
  • Construction of Small RNA cDNA Libraries for High-Throughput Sequencing
  • Focusing Mutations Within Random Libraries to Distinct Areas: Protein Domain Library Generation by Overlap Extension (PDLGO)
  • Generation of Families of Construct Variants Using Golden Gate Shuffling
  • Application of Full-Length cDNA Resources to Gain-of-Function Technology for Characterization of Plant Gene Function
  • Construction of Yeast Surface-Displayed cDNA Libraries
  • Identification of Protein/Target Molecule Interactions Using Yeast Surface-Displayed cDNA Libraries
  • SNP Discovery by Transcriptome Pyrosequencing
  • RNA-seq Analysis of Gene Expression and Alternative Splicing by Double-Random Priming Strategy
  • Generation of a Large Catalog of Unique Transcripts for Whole-Genome Expression Analysis in Non-Model Species.