cDNA Libraries : Methods and Applications /
| Autor Corporativo: | |
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| Otros Autores: | , , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2011.
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| Edición: | 1st ed. 2011. |
| Colección: | Methods in Molecular Biology,
729 |
| Materias: |
Tabla de Contenidos:
- Isolation of Fungal Infection Structures from Plant Tissue by Flow Cytometry for Cell-Specific Transcriptome Analysis
- Preparation of a High Quality cDNA Library from a Single-Cell Quantity of mRNA Using Chum-RNA
- Construction of a Full-Length cDNA Library from Castor Endosperm for High-throughput Functional Screening
- Full-Length Transcriptome Analysis Using a Bias-Free cDNA Library Prepared with the Vector-Capping Method
- Construction of Improved Yeast Two-Hybrid Libraries
- Normalization of Full-Length Enriched cDNA
- Bioinformatic Methods for Finding Differentially Expressed Genes in cDNA Libraries, Applied to the Identification of Tumour Vascular Targets
- Enzymatic Production of RNAi Libraries from cDNAs and High-Throughput Selection of Effective shRNA Expression Constructs
- Construction of Small RNA cDNA Libraries for High-Throughput Sequencing
- Focusing Mutations Within Random Libraries to Distinct Areas: Protein Domain Library Generation by Overlap Extension (PDLGO)
- Generation of Families of Construct Variants Using Golden Gate Shuffling
- Application of Full-Length cDNA Resources to Gain-of-Function Technology for Characterization of Plant Gene Function
- Construction of Yeast Surface-Displayed cDNA Libraries
- Identification of Protein/Target Molecule Interactions Using Yeast Surface-Displayed cDNA Libraries
- SNP Discovery by Transcriptome Pyrosequencing
- RNA-seq Analysis of Gene Expression and Alternative Splicing by Double-Random Priming Strategy
- Generation of a Large Catalog of Unique Transcripts for Whole-Genome Expression Analysis in Non-Model Species.