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121025s2013 xxu| s |||| 0|eng d |
| 020 |
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|a 9781627031196
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| 024 |
7 |
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|a 10.1007/978-1-62703-119-6
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a siRNA Design :
|b Methods and Protocols /
|c edited by Debra J. Taxman.
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| 250 |
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|a 1st ed. 2013.
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| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2013.
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| 300 |
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|a XIII, 392 p. 66 illus., 40 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 942
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| 505 |
0 |
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|a What Parameters to Consider and Which Software Tools to Use for Target Selection and Molecular Design of Small Interfering RNAs -- Methods for Selecting Effective siRNA Target Sequences Using a Variety of Statistical and Analytical Techniques -- Designing Functional siRNA with Reduced Off-Target Effects -- Design and Screening of siRNAs Against Highly Structured RNA Targets -- Engineering Small Interfering RNAs by Strategic Chemical Modification -- The Design, Selection and Evaluation of Highly Specific and Functional siRNA Incorporating Unlocked Nucleobase Analogs -- The Design, Preparation, and Evaluation of Asymmetric Small Interfering RNA for Specific Gene Silencing in Mammalian Cells -- Design of Nuclease-Resistant Fork-Like Small Interfering RNA (fsiRNA) -- Designing Dual-Targeting siRNA Duplexes Having Two Active Strands that Combine siRNA and MicroRNA-Like Targeting -- Strategies for Designing and Validating Immunostimulatory siRNAs -- Designing Efficient and Specific Endoribonuclease Prepared siRNAs (esiRNAs) -- Short Hairpin RNA-Mediated Gene Silencing -- Design of Lentivirally Expressed siRNAs -- Bi-Functional Short Hairpin RNA (bi-shRNA): Design and Pathway to Clinical Application -- Design and Chemical Modification of Synthetic Short shRNAs as Potent RNAi Triggers -- Production and Application of Long dsRNA in Mammalian Cells -- Design of RNAi Reagents for Invertebrate Model Organisms and Human Disease Vectors -- Construction of shRNA Expression Plasmids for Silkworm Cell Lines Using Single-Stranded DNA and Bst DNA Polymerase -- Designing Effective amiRNA and Multimeric amiRNA Against Plant Viruses -- Downregulation of Plant Genes with MiRNA Induced Gene Silencing.
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| 650 |
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0 |
|a Biotechnology.
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| 650 |
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0 |
|a Human genetics.
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| 650 |
1 |
4 |
|a Biotechnology.
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| 650 |
2 |
4 |
|a Human Genetics.
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| 700 |
1 |
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|a Taxman, Debra J.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
|