siRNA Design : Methods and Protocols /
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| Other Authors: | |
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2013.
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| Edition: | 1st ed. 2013. |
| Series: | Methods in Molecular Biology,
942 |
| Subjects: |
Table of Contents:
- What Parameters to Consider and Which Software Tools to Use for Target Selection and Molecular Design of Small Interfering RNAs
- Methods for Selecting Effective siRNA Target Sequences Using a Variety of Statistical and Analytical Techniques
- Designing Functional siRNA with Reduced Off-Target Effects
- Design and Screening of siRNAs Against Highly Structured RNA Targets
- Engineering Small Interfering RNAs by Strategic Chemical Modification
- The Design, Selection and Evaluation of Highly Specific and Functional siRNA Incorporating Unlocked Nucleobase Analogs
- The Design, Preparation, and Evaluation of Asymmetric Small Interfering RNA for Specific Gene Silencing in Mammalian Cells
- Design of Nuclease-Resistant Fork-Like Small Interfering RNA (fsiRNA)
- Designing Dual-Targeting siRNA Duplexes Having Two Active Strands that Combine siRNA and MicroRNA-Like Targeting
- Strategies for Designing and Validating Immunostimulatory siRNAs
- Designing Efficient and Specific Endoribonuclease Prepared siRNAs (esiRNAs)
- Short Hairpin RNA-Mediated Gene Silencing
- Design of Lentivirally Expressed siRNAs
- Bi-Functional Short Hairpin RNA (bi-shRNA): Design and Pathway to Clinical Application
- Design and Chemical Modification of Synthetic Short shRNAs as Potent RNAi Triggers
- Production and Application of Long dsRNA in Mammalian Cells
- Design of RNAi Reagents for Invertebrate Model Organisms and Human Disease Vectors
- Construction of shRNA Expression Plasmids for Silkworm Cell Lines Using Single-Stranded DNA and Bst DNA Polymerase
- Designing Effective amiRNA and Multimeric amiRNA Against Plant Viruses
- Downregulation of Plant Genes with MiRNA Induced Gene Silencing.