c-di-GMP Signaling : Methods and Protocols /
| Autor Corporativo: | |
|---|---|
| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2017.
|
| Edición: | 1st ed. 2017. |
| Colección: | Methods in Molecular Biology,
1657 |
| Materias: |
Tabla de Contenidos:
- Discovery of the Intracellular Signaling Molecule Cyclic di-GMP
- Enzymatic Production of C-di-GMP Using a Thermophilic Diguanylate Cyclase
- Synthesis of [32P]-c-di-GMP for Diguanylate Cyclase and Phosphodiesterase Activity Determinations
- High-performance Liquid Chromatography (HPLC)-based Detection and Quantitation of Cellular c-di-GMP
- Identification and Quantification of Cyclic Di-Guanosine Monophosphate and its Linear Metabolites by Reversed-Phase LC-MS/MS
- Detection of Cyclic Dinucleotides by STING
- Spectrophotometric and Mass Spectroscopic Methods for the Quantification and Kinetic Evaluation of In Vitro c-di-GMP Synthesis
- Gauging and Visualizing c-di-GMP Levels in Pseudomonas aeruginosa using Fluorescence-based Biosensors
- Cyclic di-GMP-responsive Transcriptional Reporter Bioassays in Pseudomonas aeruginosa
- Live flow cytometry analysis of c-di-GMP levels in Single Cell Populations
- Experimental Detection and Visualization of the Extracellular Matrix in Macrocolony Biofilms
- Congo Red Stain Identifies Matrix Overproduction and is an Indirect Measurement for c-di-GMP in many Species of Bacteria
- Type IV pili-dependent Motility as a Tool to Determine the Activity of c-di-GMP Modulating Enzymes in Myxococcus xanthus
- Using Light-Activated Enzymes for Modulating Intracellular c‑di-GMP Levels in Bacteria
- Analysis of c-di-GMP Levels Synthesized by a Photoreceptor Protein in Response to Different Light Qualities using an in vitro Enzymatic Assay
- Probing the Role of Cyclic di-GMP Signalling Systems in Disease using Chinese Radish
- Contribution of Cyclic di-GMP in the Control of Type III and Type VI Secretion in Pseudomonas aeruginosa
- Semi-quantitative Analysis of the Red, Dry, and Rough Colony Morphology of Salmonella enterica serovar Typhimurium and Escherichia coli using Congo Red
- Fluorescent 2-aminopurine c-di-GMP and GpG analogs as PDE Probes
- Measuring Cyclic Diguanylate (c-di-GMP)-specific Phosphodiesterase Activity using the MANT-c-di-GMP Assay
- Determining Phosphodiesterase Activity (Radioactive Assay)
- Determining Diguanylate Cyclase Activity (Radioactive Assay)
- Detection of c-di-GMP-responsive DNA Binding
- Use of Nonradiochemical DNAse Footprinting to Analyze c-di-GMP Modulation of DNA-binding Proteins
- Detection of Cyclic di-GMP Binding Proteins Utilizing a Biotinylated Cyclic di-GMP Pulldown Assay
- Probing Protein-protein Interactions with Genetically Encoded Photo-activatable Crosslinkers
- Identification of c-di-AMP-Binding Proteins Using Magnetic Beads
- Pulldown with a c-di-GMP-specific Capture Compound Coupled to Mass Spectrometry as a Powerful Tool to Identify Novel Effector Proteins
- Identification of c-di-GMP-responsive Riboswitches
- Isothermal Titration Calorimetry to Determine Apparent Dissociation Constants (Kd) and Stoichiometry of Interaction (n) of c-di-GMP Binding Proteins
- Targeting c-di-GMP Signaling, Biofilm Formation and Bacterial Motility With Small Molecules
- Discovering Selective Diguanylatecyclases Inhibitors: From PleD to Discrimination of the Active Site of cyclic-di-GMP Phosphodiesterases
- High throughput Screening for Compounds that Modulate the Cellular c-di-GMP Level in Bacteria
- Genetic Tools to Study c-di-GMP-dependent Signaling in Pseudomonas aeruginosa. .