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150604s2015 xxu| s |||| 0|eng d |
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|a 9781493926947
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| 024 |
7 |
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|a 10.1007/978-1-4939-2694-7
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Western Blotting :
|b Methods and Protocols /
|c edited by Biji T. Kurien, R. Hal Scofield.
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| 250 |
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|a 1st ed. 2015.
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| 260 |
# |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2015.
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| 300 |
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|a XIV, 509 p. 140 illus., 65 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1312
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| 505 |
0 |
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|a The Early Days of Blotting -- Origins of Protein Blotting -- Western Blotting: Remembrance of Things Past -- Simian Virus 40 and Protein Transfer -- Western Blotting: An Introduction -- From Little Helpers to Automation -- Spectrophotometric Methods to Determine Protein Concentration -- Solubilization of Proteins: The Importance of Lysis Buffer Choice -- Simultaneous Immunoblotting Analysis with Activity Gel Electrophoresis and 2-D Gel Electrophoresis -- Diffusion Blotting: A Rapid and Simple Method for Production of Multiple Blots from a Single Gel -- Multiple Immunoblots by Passive Diffusion of Proteins from a Single SDS-PAGE Gel -- Slice Blotting -- Localizing Proteins by Tissue Printing -- Dot-Immunobinding Assay (Dot-Iba) -- Analysis of Antibody Clonotype by Affinity Immunoblotting -- Glycosaminoglycan Blotting and Detection after Electrophoresis Separation -- A Well-Based Reverse-Phase Protein Array of Formalin-Fixed Paraffin-Embedded Tissue -- Quantitative Computerized Western Blotting in Detail -- Cationic Electrophoresis and Eastern Blotting -- A Miniaturized Blotting System for Simultaneous Detecting of Different Autoantibodies -- Proteomic Expressional Profiling of a Paraffin Embedded Tissue by Multiplex Tissue Immunoblotting -- Post-Staining Electroblotting for Efficient and Reliable Peptide Blotting -- Multistrip Western Blotting: A Tool for Comparative Quantitative Analysis of Multiple Proteins -- Western Blotting using PVDF Membranes and Its Downstream Applications -- Blotting from PhastGel to Membranes by Ultrasound -- Western Blotting of High and Low Molecular Weight Proteins Using Heat -- Membrane Strip Affinity Purification of Autoantibodies -- Strip Immunoblotting of Multiple Antigenic Peptides -- Double-Blotting: A Solution to the Problem of Non-Specific Binding of Secondary Antibodies in Immunoblotting Procedures -- Method for Resolution and Western Blotting of Very Large Proteins Using Agarose Electrophoresis -- Immunodetection of P-Selectin Using an Antibody to its C-Terminal Tag -- Improvements and Variants of the Mutliple Antigen Blot Assay-MABA: An Immunoenzymatic Technique for Simultaneous Antigen and Antibody Screening -- Blotting from Immobilized pH Gradient Gels: Application to Total Cell Lysates -- Immunoprecipitation: Western Blot for Proteins of Low Abundance -- Native Electrophoresis and Western Blot Analysis (NEWeB): Methods and Applications -- Shift-Western Blotting: Separate Analysis of Protein and DNA from Protein-DNA Complexes -- Grid-Immunoblotting -- Detection and Quantification of Protein-Protein Interactions by Far-Western Blotting -- Western Blot Analysis of Adhesive Interactions under Fluid Shear Conditions: The Blot Rolling Assay -- Centrifuge Blotting -- Blotting of Coomassie Blue Stained Proteins from PAGE Gels to Transparencies -- B Cell ELISPOT: For the Identification of Antigen-Specific Antibody Secreting Cells -- T Cell ELISPOT: For the Identification of Specific Cytokine-Secreting T Cells -- Membrane Microplates for One- and Two-Color ELISPOT and FLUOROSPOT Assays -- SDS PAGE to Immunoblot in One Hour -- Single Cell Western Blotting -- Protein Detection by Simple Western™ Analysis -- Western Blotting Using Microfluidics -- Two-Dimensional Gel-Based Protein Standardization Verified by Western Blot Analysis -- Fingerprint Deposition of Nitrocellulose and Polyvinylidene Difluoride Membranes Using Alkaline Phosphatase -- Other Notable Protein Blotting Methods: A Brief Review. .
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| 650 |
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|a Proteins .
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| 650 |
1 |
4 |
|a Protein Science.
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| 700 |
1 |
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|a Kurien, Biji T.
|e editor.
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| 700 |
1 |
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|a Scofield, R. Hal.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
|