cAMP Signaling : Methods and Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Zaccolo, Manuela. (Editor)
Format: eBook
Language:English
Published: New York, NY : Springer New York : Imprint: Humana, 2015.
Edition:1st ed. 2015.
Series:Methods in Molecular Biology, 1294
Subjects:
Table of Contents:
  • Simultaneous Assessment of cAMP Signaling Events in Different Cellular Compartments Using FRET-Based Reporters
  • Recording Intracellular cAMP Levels with EPAC-Based FRET Sensors by Fluorescence Lifetime Imaging
  • A Novel Approach Combining Real-Time Imaging and the Patch-Clamp Technique to Calibrate FRET-Based Reporters for cAMP in Their Cellular Microenvironment
  • Structure-Based, In Silico Approaches for the Development of Novel cAMP FRET Reporters
  • Automated Image Analysis of FRET Signals for Subcellular cAMP Quantification
  • Channel-Based Reporters for cAMP Detection
  • Imaging Sub-Plasma Membrane cAMP Dynamics with Fluorescent Translocation Reporters
  • Adenoviral Transduction of FRET-Based Biosensors for cAMP in Primary Adult Mouse Cardiomyocytes
  • Generation of Transgenic Mice Expressing FRET Biosensors
  • Photoactivatable Adenylyl Cyclase (PACs) as a Tool to Study cAMP Signaling In Vivo: An Overview
  • Selective Disruption of the AKAP Signaling Complexes
  • Screening for Small Molecule Disruptors of AKAP-PKA Interactions
  • Structure-Based Bacteriophage Screening for AKAP-Selective PKA Regulatory Subunit Variants
  • A Yeast-Based High-Throughput Screen for Modulators of Phosphodiesterase Activity
  • Separation of PKA and PKG Signaling Nodes by Chemical Proteomics
  • Development of Computational Models of cAMP Signaling.