Whole Genome Amplification : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Kroneis, Thomas. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2015.
Edición:1st ed. 2015.
Colección:Methods in Molecular Biology, 1347
Materias:
Tabla de Contenidos:
  • Principles of Whole Genome Amplification.- Bias in Whole Genome Amplification: Causes and Considerations.- The Single-Cell Lab or How to Perform Single-Cell Molecular Analysis
  • Sample Preparation Methods Following Cell Search Approach Compatible of Single-Cell Whole Genome Amplification: An Overview
  • Deterministic Whole Genome Amplification of Single Cells
  • Construction of a DNA Library on Micro beads Using Whole Genome Amplification.- Heat-Induced Fragmentation and Adapter-Assisted Whole Genome Amplification Using Genome Plex® Single Cell Whole Genome Amplification Kit (WGA4).- Whole Genome Amplification by Isothermal Multiple Strand Displacement Using Phi29 DNA Polymerase.- Using Multiplex-PCR for Assessing the Quality of Whole Genome Amplified DNA.- Quality Control of Isothermal Amplified DNA Based on Short Tandem Repeat Analysis.- Laser-Micro dissection of FFPE Tissue Areas and Subsequent Whole Genome Amplification by Ampli1TM.- Whole Genome Amplification from Blood Spot Samples.- Analysis of Whole Mitogenomes from Ancient Samples.- Copy Number Variation Analysis by Array Analysis of Single Cells Following Whole Genome Amplification.- Whole Genome Amplification in Genomic Analysis of Single Circulating Tumor Cells.- Whole Genome Amplification of Labeled Viable Single Cells Suited for Array Comparative Genomic Hybridization.- Low-Volume On-Chip Single-Cell Whole Genome Amplification for Multiple Subsequent Analyses.- Detection and Characterization of Circulating Tumor Cells by the Cell Search Approach.