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100301s2005    ne |    s    |||| 0|eng d | 
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|a 9781402037351 
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|a 10.1007/1-4020-3735-X 
  |2 doi 
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica 
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|a Lotus japonicus Handbook 
  |c edited by Antonio J. Márquez. 
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| 250 | 
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|a 1st ed. 2005. 
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|a Dordrecht : 
  |b Springer Netherlands : 
  |b Imprint: Springer, 
  |c 2005. 
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| 300 | 
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|a XXII, 384 p. 
  |b online resource. 
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|a text 
  |b txt 
  |2 rdacontent 
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|a computer 
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  |2 rdamedia 
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|a online resource 
  |b cr 
  |2 rdacarrier 
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|a Lotus japonicus's a model system -- Lotus-related species and their agronomic importance -- Drought and saline stress -- Methods for studying nodule development and function -- A procedure for in vitro nodulation studies -- Arbuscular mycorrhiza -- Concurrent visualization of gusA and lacZ reporter gene expression -- Embedding root and nodule tissue in plastic (BMM) -- RNA isolation using phase extraction and LiCl precipitation -- RNA isolation using CsCl gradients -- 96-Well DNA isolation method -- Genome structure analysis -- Transcriptome analysis using cDNA arrays -- Lotus japonicus expression database -- Procedures for mass spectrometric proteome analysis -- Isolation of peribacteroid membranes for proteome analysis -- Metabolome analysis using GC-MS -- Mutagenesis -- A mutant catalogue of Lotus japonicus -- Tilling -- Ds gene-tagging -- Mapping and map-based cloning -- An in silico strategy towards the development of legume genome anchor markers using comparative sequence analysis -- Information transfer: mapping and cloning in other legumes -- Agrobacterium-mediated in vitro transformation -- Induction of hairy roots for symbiotic gene expression studies -- Transformation-regeneration procedure for Lotus japonicus -- Agrobacterium rhizogenes pRi TL-DNA integration system: a gene vector for Lotus japonicus transformation -- Vectors for reverse genetics and expression analysis -- Nitrate assimilation: Influence of nitrogen supply -- Nitrate and ammonium assimilatory enzymes -- Transgenic plants affected in nitrate assimilation -- Secondary metabolite profiling -- Phenolic Compounds: extraction and analysis -- Elicitation of isoflavan phytoalexins -- An update of work published on Lotus japonicus. 
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|a Plant science. 
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| 650 | 
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|a Botany. 
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| 650 | 
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|a Plant genetics. 
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| 650 | 
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|a Microbiology. 
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| 650 | 
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|a Plant biochemistry. 
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| 650 | 
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|a Agriculture. 
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| 650 | 
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|a Plant physiology. 
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|a Plant Sciences. 
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| 650 | 
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|a Plant Genetics and Genomics. 
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| 650 | 
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|a Microbiology. 
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| 650 | 
2 | 
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|a Plant Biochemistry. 
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| 650 | 
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|a Agriculture. 
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| 650 | 
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|a Plant Physiology. 
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| 700 | 
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|a Márquez, Antonio J. 
  |e editor. 
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| 710 | 
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|a SpringerLink (Online service) 
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| 773 | 
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|t Springer eBooks 
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|u https://doi.org/10.1007/1-4020-3735-X 
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