Lotus japonicus Handbook

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Márquez, Antonio J. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Dordrecht : Springer Netherlands : Imprint: Springer, 2005.
Edición:1st ed. 2005.
Materias:
Acceso en línea:https://doi.org/10.1007/1-4020-3735-X
Tabla de Contenidos:
  • Lotus japonicus’s a model system
  • Lotus-related species and their agronomic importance
  • Drought and saline stress
  • Methods for studying nodule development and function
  • A procedure for in vitro nodulation studies
  • Arbuscular mycorrhiza
  • Concurrent visualization of gusA and lacZ reporter gene expression
  • Embedding root and nodule tissue in plastic (BMM)
  • RNA isolation using phase extraction and LiCl precipitation
  • RNA isolation using CsCl gradients
  • 96-Well DNA isolation method
  • Genome structure analysis
  • Transcriptome analysis using cDNA arrays
  • Lotus japonicus expression database
  • Procedures for mass spectrometric proteome analysis
  • Isolation of peribacteroid membranes for proteome analysis
  • Metabolome analysis using GC-MS
  • Mutagenesis
  • A mutant catalogue of Lotus japonicus
  • Tilling
  • Ds gene-tagging
  • Mapping and map-based cloning
  • An in silico strategy towards the development of legume genome anchor markers using comparative sequence analysis
  • Information transfer: mapping and cloning in other legumes
  • Agrobacterium-mediated in vitro transformation
  • Induction of hairy roots for symbiotic gene expression studies
  • Transformation-regeneration procedure for Lotus japonicus
  • Agrobacterium rhizogenes pRi TL-DNA integration system: a gene vector for Lotus japonicus transformation
  • Vectors for reverse genetics and expression analysis
  • Nitrate assimilation: Influence of nitrogen supply
  • Nitrate and ammonium assimilatory enzymes
  • Transgenic plants affected in nitrate assimilation
  • Secondary metabolite profiling
  • Phenolic Compounds: extraction and analysis
  • Elicitation of isoflavan phytoalexins
  • An update of work published on Lotus japonicus.