|
|
|
|
| LEADER |
04641nam a22003255i 4500 |
| 001 |
978-1-4612-2056-5 |
| 005 |
20191026161226.0 |
| 007 |
cr nn 008mamaa |
| 008 |
110824s1996 xxu| s |||| 0|eng d |
| 020 |
|
|
|a 9781461220565
|
| 024 |
7 |
|
|a 10.1007/978-1-4612-2056-5
|2 doi
|
| 040 |
|
|
|a Sistema de Bibliotecas del Tecnológico de Costa Rica
|
| 100 |
1 |
|
|a Rosenberg, Ian M.
|e author.
|
| 245 |
1 |
0 |
|a Protein Analysis and Purification
|b Benchtop Techniques /
|c by Ian M. Rosenberg.
|
| 250 |
|
|
|a 2nd ed. 1996.
|
| 260 |
# |
# |
|a Boston, MA :
|b Birkhäuser Boston :
|b Imprint: Birkhäuser,
|c 1996.
|
| 300 |
|
|
|a XXVI, 520 p.
|b online resource.
|
| 336 |
|
|
|a text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a computer
|b c
|2 rdamedia
|
| 338 |
|
|
|a online resource
|b cr
|2 rdacarrier
|
| 505 |
0 |
|
|a 1 An Overview of This Manual -- Kits, Cores, and Computers -- How to Use This Book -- Basic Laboratory Equipment -- Laboratory Automation -- Beyond Protein Analysis and Purification -- 2 Protein Structure -- A. The Amino Acids -- B. The Four Levels of Protein Structure -- C. Chemical Characteristics of Proteins -- 3 Tracking the Target Protein -- A. Labeling Cells and Proteins -- B. Lysis—Preparation of the Cell Free Extract -- C. Principles of Immunoprecipitation -- D. Additional Methods to Identify Associated Proteins -- 4 Electrophoretic Techniques -- to Polyacrylamide Gel Electrophoresis (PAGE) -- A. Preparation of SDS-Polyacrylamide Gels -- B. 2-Dimensional (2-D) Gel Systems -- D. Recovery of Proteins from the Gel -- E. Identification of Enzyme Activity in Polyacrylamide Gels -- 5 Getting Started with Protein Purification -- A. Making a Cell Free Extract -- B. Protein Quantitation -- C. Manipulating Proteins in Solution -- D. Precipitation Techniques -- 6 Membrane Proteins -- A. Peripheral Membrane Proteins -- B. Integral Membrane Proteins -- C. Detergents -- 7 Transfer and Detection of Proteins on -- Membrane Supports -- A. Transfer of Proteins to Membrane Supports -- B. Staining the Blot -- C. Recovery of Proteins from the Blot -- D. Detection of the Target Protein -- 8 Identification of the Target Protein -- A. Peptide Mapping -- B. Enzymatic Cleavage of Proteins -- C. Chemical Cleavage -- D. Microsequencing from PVDF Membranes -- MS Basics -- Protein Identification by MS -- Protein Database Searches -- The Rise of Biological Databases -- 9 Identifying and Analyzing Posttranslational -- Modifications -- A. Glycosylation -- B. Phosphorylation -- C. Lipid Modification of Proteins -- D. Selected Modifications -- 10 Chromatography -- A. Important Terminology Used in Chromatography -- B. Gel Filtration Chromatography -- C. Introduction to HPLC -- D. Ion Exchange Chromatography: Separation on the Basis of Charge -- E. Hydrophobic Interaction Chromatography (HIC) -- Flow Rate -- 11 Recombinant Protein Techniques -- Recombinant Protein for Antibody Production -- Protein for Biochemical or Cell Biological Studies -- A. In vitro Transcription and Translation -- B. Recombinant Gene Products in E. coli: Expression, Identification and Characterization -- D. Expression of Foreign Proteins in Eukaryotic Cells -- Appendices -- A. Safety Considerations -- First Aid: Emergency Procedures -- B. Antibody Preparation -- Production of Polyclonal Antisera in Rabbits -- Purification of Antibody Using Protein A Affinity Columns -- Numbering Mice -- Molarity -- Choosing and Preparing Buffers -- Common Laboratory Solutions -- Extinction Coefficients -- D. Nucleic Acids -- Spectrophotometric Conversions -- DNA/Protein Conversions -- Oligonucleotide Concentrations -- RNA Precipitation -- E. Modifications and Motifs -- Nomenclature -- Protein Modification Sequences -- Protein Kinase Recognition Sequence Motifs -- Subcellular Localization Motifs -- Protein Databases -- F. Centrifugation -- Nomogram -- General Purpose Centrifuge Rotors -- Ultracentrifuge Rotors -- G. Proteases and Proteolytic Enzyme Inhibitors -- Commonly Used Proteases -- Protease Inhibitors -- H. Radioactivity -- Manual and Machine Film Processing -- L Tissue Culture -- Transwell Permeable Supports -- J. Miscellaneous -- Unit Prefixes -- The Greek Alphabet -- Abbreviations -- HPLC Pump Pressure Conversion -- Dipeptide Masses -- Mass Differences Considered in Molecular Weight Analysis of Proteins -- K. List of Suppliers, Vendors, Manufacturers.
|
| 650 |
|
0 |
|a Proteins .
|
| 650 |
|
0 |
|a Biochemistry.
|
| 650 |
|
0 |
|a Life sciences.
|
| 650 |
1 |
4 |
|a Protein Science.
|
| 650 |
2 |
4 |
|a Biochemistry, general.
|
| 650 |
2 |
4 |
|a Life Sciences, general.
|
| 710 |
2 |
|
|a SpringerLink (Online service)
|
| 773 |
0 |
|
|t Springer eBooks
|
| 856 |
4 |
0 |
|u https://doi.org/10.1007/978-1-4612-2056-5
|