Regulatory Non-Coding RNAs Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Carmichael, Gordon G. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2015.
Edición:1st ed. 2015.
Colección:Methods in Molecular Biology, 1206
Materias:
Acceso en línea:https://doi.org/10.1007/978-1-4939-1369-5
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245 1 0 |a Regulatory Non-Coding RNAs  |b Methods and Protocols /  |c edited by Gordon G. Carmichael. 
250 |a 1st ed. 2015. 
260 # # |a New York, NY :  |b Springer New York :  |b Imprint: Humana,  |c 2015. 
300 |a X, 181 p. 39 illus., 10 illus. in color.  |b online resource. 
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490 1 |a Methods in Molecular Biology,  |v 1206 
505 0 |a Diverse Functions and Mechanisms of Mammalian Long Non-Coding RNAs -- Sequence-Defined Oligoaminoamides for the Delivery of siRNAs -- A Biochemical Approach to Identify Direct MicroRNA Targets -- Native Gel Analysis for Mammalian MicroRNPs Assembled from Pre-MicroRNAs -- Identification and Validation of miRNA Target Sites within Non-Traditional miRNA Targets -- Fractionation of Non-Polyadenylated and Ribosomal-Free RNAs from Mammalian Cells -- Affinity-Purification of Long Noncoding RNA−Protein Complexes from Formaldehyde-Cross linked Mammalian Cells -- RNA Pull down Protocol for In Vitro Detection and Identification of RNA-Associated Proteins -- nRIP-seq: A Technique to Identify RNA Targets of an RNA Binding Protein on a Genome-Wide Scale -- Analysis of the Subcellular Distribution of RNA by Fluorescence In Situ Hybridization -- RNA Fluorescence In Situ Hybridization in Cultured Mammalian Cells -- Locating RNAs In Situ with FISH-STIC Probes -- Rapid and Efficient Elimination of Specific Nuclear Noncoding RNAs in Mammalian Cells with Antisense Oligonucleotides -- On Using Magnesium and Potassium Ions in RNA Experiments -- Studying the Affinity, Kinetic Stability and Specificity of RNA/Protein Interactions: SINE ncRNA/Pol II Complexes as a Model System. 
650 0 |a Human genetics. 
650 1 4 |a Human Genetics. 
700 1 |a Carmichael, Gordon G.  |e editor. 
710 2 |a SpringerLink (Online service) 
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856 4 0 |u https://doi.org/10.1007/978-1-4939-1369-5