Nuclear G-Protein Coupled Receptors Methods and Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Allen, Bruce G. (Editor), Hébert, Terence E. (Editor)
Format: eBook
Language:English
Published: New York, NY : Springer New York : Imprint: Humana, 2015.
Edition:1st ed. 2015.
Series:Methods in Molecular Biology, 1234
Subjects:
Online Access:https://doi.org/10.1007/978-1-4939-1755-6
Table of Contents:
  • Studies of Intracellular Angiotensin II
  • Single-Cell Microinjection Coupled to Confocal Microscopy to Characterize Nuclear Membrane Receptors in Freshly Isolated Cardiomyocytes
  • Design and Application of Light-Activated Probes for Cellular Signaling
  • Using Caged Ligands to Study Intracrine Endothelin Signalling In Intact Cardiac Myocytes
  • Quantification of Catecholamine Uptake in Adult Cardiac Myocytes
  • Characterization of the Interaction Between the Prostaglandin D2 DP1 Receptor and the Intracellular L-Prostaglandin D Synthase
  •  Isolation and Study of Cardiac Nuclei from Canine Myocardium and Adult Ventricular Myocytes
  • High Resolution Imaging and Function of Nuclear G Protein-Coupled Receptors (GPCRs)
  • Biochemical Fractionation of Membrane Receptors in the Nucleus
  • Functional G Protein-Coupled Receptors on Nuclei From Brain and Primary Cultured Neurons
  • Automated Microscopy of Cardiac Myocyte Hypertrophy: A Case Study on the Role of Intracellular a-Adrenergic Receptors
  • Measuring Intra-Nuclear and Nuclear Envelope [Ca2+] vs. Cytosolic [Ca2+]
  • Assessing GPCR and G Protein Signaling to the Nucleus in Live Cells Using Fluorescent Biosensors
  • Tandem Affinity Purification to Identify Cytosolic and Nuclear Gbg-Interacting Proteins
  • Examining the Effects of Nuclear GPCRs on Gene Expression Using Isolated Nuclei
  • Trafficking and Function of GPCRs in the Endosomal Compartment.