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02357nam a22002895i 4500 |
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978-1-4939-2772-2 |
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20191026161457.0 |
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cr nn 008mamaa |
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150715s2015 xxu| s |||| 0|eng d |
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|a 9781493927722
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|a 10.1007/978-1-4939-2772-2
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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|a Hedgehog Signaling Protocols
|c edited by Natalia A. Riobo.
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| 250 |
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|a 2nd ed. 2015.
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| 260 |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2015.
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| 300 |
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|a XVI, 200 p. 42 illus., 28 illus. in color.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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|a online resource
|b cr
|2 rdacarrier
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|a Methods in Molecular Biology,
|v 1322
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|a Controlled Delivery of Sonic Hedgehog with a Heparin-Based Coacervate -- In Vivo Imaging of Hedgehog Transport in Drosophila Epithelia -- Modeling Hedgehog Signaling Through Flux-Saturated Mechanism -- Evaluating the Activity of Smoothened Toward G Proteins Using [35S] Guanosine 5’-(3-O-thio)triphosphate ([35S]GTPγS) -- Analysis of Smoothened Phosphorylation and Activation in Cultured Cells and Wing Discs of Drosophila -- Investigation of Protein-Protein Interactions and Conformational Changes in Hedgehog Signaling Pathway by FRET -- Luciferase Reporter Assays to Study Transcriptional Activity of HH Signaling in Normal and Cancer Cells -- Measuring Expression Levels of Endogenous Gli Genes by Immunoblotting and Real-Time PCR -- Quantitative Immunoblotting of Endogenous Hedgehog Pathway Components -- Measuring Gli2 Phosphorylation by Selected Reaction Monitoring Mass Spectrometry -- Rapid Screening of Gli2/3 Mutants Using the Flp-In System -- Insights into Gli Factors Ubiquitylation Methods -- Determination of Acetylation of the Gli Transcription Factors -- Efficient Detection of Indian Hedgehog During Endochondral Ossification by Whole Mount Immunofluorescence -- Methods for Detection of Ptc1-Driven LacZ Expression in Adult Mouse Skin -- Determination and Analysis of Cellular Metabolic Changes by Non-Canonical Hedgehog Signaling.
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|a Proteins .
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| 650 |
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|a Protein Science.
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| 700 |
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|a Riobo, Natalia A.
|e editor.
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| 710 |
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|a SpringerLink (Online service)
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1007/978-1-4939-2772-2
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