Antigen Processing Methods and Protocols /
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| Other Authors: | |
| Format: | eBook |
| Language: | English |
| Published: |
New York, NY :
Springer New York : Imprint: Humana,
2019.
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| Edition: | 2nd ed. 2019. |
| Series: | Methods in Molecular Biology,
1988 |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/978-1-4939-9450-2 |
Table of Contents:
- Purification of Large Cytosolic Proteases for In Vitro Assays: 20S and 26S Proteasomes
- Analysis of Proteasome Generated Antigenic Peptides by Mass Spectrometry
- Trimming of MHC Class I Ligands by ERAP Aminopeptidases
- Peptide Trimming for MHC Class I Presentation by Endoplasmic Reticulum Aminopeptidases
- Testing the Impact of Protease Inhibitors in Antigen Presentation Assays
- In Vitro Studies of MHC Class I Peptide Loading and Exchange
- Measuring Synthesis and Degradation of MHC Class I Molecules
- Biochemical Analysis of Naturally Processed Antigenic Peptides Presented by MHC Class I Molecules
- A SIINFEKL Based System to Measure MHC Class I Antigen Presentation Efficiency and Kinetics
- Purification and Identification of Naturally Presented MHC Class I and II Ligands
- Identification of MHC Ligands and Establishing MHC Class I Peptide Motifs
- Quantitating MHC Class I Ligand Production and Presentation using TCR-Like Antibodies
- Identifying Antigens Recognized by Cytolytic T Lymphocytes on Tumors
- A Flow Cytometry-Based Approach to Unravel Viral Interference with the MHC Class I Antigen Processing and Presentation Pathway
- Evaluating CD8+ T Cell Responses In Vitro
- Recombinant Poxviruses: Versatile Tools for Immunological Assays
- Monitoring the Intracellular Routing of Internalized Antigens by Immunofluorescence Microscopy
- Monitoring MHC Ubiquitination by MARCH Ubiquitin Ligases
- Monitoring MHC-II Endocytosis and Recycling using Cell-Surface Protein Biotinylation-Based Assays
- Monitoring Protein Endocytosis and Recycling using FACS-Based Assays
- In Vitro Digestion with Proteases Producing MHC Class II Ligands
- Assembly, Intracellular Transport and Release of MHC Class II Peptide Receptors
- Pulse-Chase Analysis for Studies of MHC Class II Biosynthesis, Maturation, and Peptide Loading
- Studying MHC Class II Peptide Loading and Editing In Vitro
- Monitoring Antigen Processing for MHC Presentation via Macroautophagy
- Identification of Human Antigen-Specific CD4+ T-Cells with Peptide-MHC Multimer Technologies
- Production of Antigen Specific Human CD4+ T Cell Lines and Clones
- Monitoring Dendritic Cell Activation and Maturation
- Studying MHC Class II Presentation of Immobilized Antigen by B Lymphocytes
- Analyzing Antigen Recognition by Natural Killer T Cells.