SV40 Protocols

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Raptis, Leda. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 2001.
Edition:1st ed. 2001.
Series:Methods in Molecular Biology, 165
Subjects:
Online Access:https://doi.org/10.1385/1592591175
Table of Contents:
  • Propagation of Wild-Type and Mutant SV40
  • Propagation and Assay of the JC Virus
  • Propagation and Assay of BK Virus
  • Molecular Methods for Identification and Genotyping of BK Virus
  • Methods for Studying Interactions Between Simian Virus 40 T-Antigen and the Viral Origin of Replication
  • Cell-Free Transcription of SV40
  • Quantitation and Structural Analysis of SV40 RNAs
  • Generation of Recombinant SV40 Vectors for Gene Transfer
  • Production of SV40 Proteins in Insect Cells and In Vitro Packaging of Virions and Pseudovirions
  • Using Retroviral Vectors to Express SV40 Tumor Antigens
  • Neoplastic Transformation Assays
  • Methods to Assess Inhibition of Adipocyte Differentiation by SV40 Large T Antigen
  • SV40-Mediated Immortalization
  • Biology of p53 and SV40 Large T Association
  • Binding of SV40 Large T Antigen to the Retinoblastoma Susceptibility Gene Product and Related Proteins
  • In situ Electroporation for the Measurement of c-Ras Activation by SVLT
  • Role of SV40 Small t in Cell Lysis, Transformation, and Signaling
  • Cytotoxic T Lymphocytes in SV40 Infections
  • Detection of SV40 DNA Sequences in Human Tissue
  • Guide to Techniques in Creating Transgenic Mouse Models Using SV40 T Antigen.