Metastasis Research Protocols

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Brooks, Susan A. (Editor ), Schumacher, Udo. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2001.
Edición:1st ed. 2001.
Colección:Methods in Molecular Medicine, 57
Materias:
Acceso en línea:https://doi.org/10.1385/1592591361
Tabla de Contenidos:
  • Detection Methods for Cellular Markers of Metastatic Potential
  • Histopathological Assessment of Metastasis
  • Basic Immunocytochemistry for Light Microscopy
  • Multiple Labeling Techniques for Fluorescence Microscopy
  • Lectin Histochemistry to Detect Altered Glycosylation in Cells and Tissues
  • Immunocytochemical Detection and Characterization of Individual Micrometastatic Tumor Cells
  • Understanding Metastasis Through Integrin Expression
  • Surrogate Markers of Angiogenesis and Metastasis
  • Measurement of Microvessel Density in Primary Tumors
  • Assessment of Cellular Proliferation by Calculation of Mitotic Index and by Immunohistochemistry
  • Flow Cytometric Measurement of Cell Proliferation
  • SDS -PAGE and Western Blotting Techniques
  • Assessment of Gelatinases (MMP-2 and MMP-9 by Gelatin Zymography
  • Genetic Aspects of Metastasis
  • In Situ Hybridization to Localize mRNAs
  • Fluorescence itIn Situ Hybridization
  • Comparative Genomic Hybridization
  • RNA Purification and Quantification Methods
  • Northern and Southern Blotting and the Polymerase Chain Reaction to Detect Gene Expression
  • Methylation Analysis of CpG Islands
  • Detection of MetastaticTumor Cells in Blood by Reverse Transcriptase-Polymerase Chain Reaction
  • Differential Display
  • Mathematical Modeling of Metastasis
  • Predictive Mathematical Modeling in Metastasis.